Main menu

Whole genome Nanopore DNA analysis shows that chronic corticosterone supplementation results in altered sperm DNA methylation.


Introduction

  • Corticosterone (CORT) is the main stress hormone in mice.
  • CORT supplementation models Hypothalamic-Pituitary-Adrenal (HPA) axis activation, the neuroendocrine system that regulates stress but also plays a role in digestion, emotions, immune system and metabolism.
  • 4 weeks of CORT supplementation in male mice lead to altered sperm small non coding RNA (sncRNA) changes in F0.
  • Paternal CORT leads to behavioral changes in their offspring (F1 and F2). Including alterations in memory, anxiety and depression phenotypes.
  • Paternal CORT leads to expression changes in the paternal imprinted gene Igf2 in hippocampus of F1 and F2 male offspring and F1 female offspring.

Download the PDF

Getting started

Buy a MinION starter pack Nanopore store Sequencing service providers Channel partners

Nanopore technology

Subscribe to Nanopore updates Resources and publications What is the Nanopore Community

About Oxford Nanopore

News Company timeline Sustainability Leadership team Media resources & contacts For investors For partners Working at Oxford Nanopore Current vacancies Commercial information BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
English flag