Directly detect and phase genomic methylation with high reproducibility and low bias

Epigenetic modifications are chemical changes that can alter phenotype without altering nucleotide sequence. The most well-characterised and widely studied epigenetic modification in mammalian genomes is 5mC DNA methylation. With methylation playing an important role in regulating gene expression, aberrant methylation in gene promoters is often associated with disease.

In this webinar hear Philipp share how nanopore sequencing can be used for the direct detection of intact methylation alongside nucleotide sequence.

You can expect to learn:

• The best way to call 5mC methylation using nanopore sequence data and the read length and coverage requirements
• The 5mC methylation calling capabilities using nanopore sequence data, in comparison to publicly available bisulphite sequencing datasets
• How to run haplotype-resolved methylation analysis

Meet our speaker

Philipp Rescheneder works as a bioinformatician in the Applications team at Oxford Nanopore. In his current role he identifies, benchmarks and adapts the most appropriate tools and workflows for analysing Oxford Nanopore data in the context of a wide range of applications, including variant calling, assembly, and methylation calling. Before joining Oxford Nanopore, his research focused on developing computational tools for efficiently aligning noisy long and short next-generation sequencing reads.