Utilising nanopore direct RNA sequencing of blood from patients with sepsis for discovery of co- and post-transcriptional disease biomarkers
He and Ganesamoorthy et al. used Oxford Nanopore direct RNA sequencing to gain insights into transcriptional and post-transcriptional regulation in sepsis. They were able to capture full-length mRNA from blood samples, revealing gene expression, poly(A) tail length, and isoform-level changes. Oxford Nanopore sequencing provided insights beyond those possible with standard techniques, advancing understanding of infection biology and paving the way for RNA-based diagnostics in the future.
'Our results suggest that integrating nanopore direct RNA sequencing into research workflows could significantly enhance insights into RNA regulation and gene expression, providing valuable contributions to understanding disease mechanisms'
He and Ganesamoorthy et al. 2025
Sample type: human blood