Michael Micorescu
Tombo: detection of non-standard nucleotides using raw nanopore signal
About Michael Micorescu
Michael’s graduate and postdoctoral work at Portland State University and Oregon Health Sciences University focused on the biology of gene expression and regulation. Michael then worked as a senior scientific specialist for a leading next-generation sequencing company. He joined Oxford Nanopore Technologies in September 2014 as Technical Applications Manager. He is currently responsible for overseeing US technical support operations.
Abstract
Tombo is a suite of tools for the identification of modified nucleotides from nanopore sequencing data and visualization of raw nanopore signal. Tombo provides three methods for the investigation of DNA base modifications: specific alternative base detection, canonical (control) sample comparison and de novo canonical model comparison. In addition, Tombo includes a workflow for processing nanopore signal generated by direct RNA sequencing. The advantages and requirements for each detection method will be discussed.
Michael Micorescu