Rapid culture-independent diagnostics of infectious diseases by Oxford Nanopore genomic sequencing
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Background
Infectious diseases affect millions of people every year. Particular virulent and multi-drug resistant agents are increasingly responsible for infections with ever-expanding complexities. Therefore, it is critical to establish rapid, efficient and accurate diagnostic methods to apply the proper treatment as soon as possible.
Purpose
Optimization and application of Oxford Nanopore long-range DNA sequencing, as a rapid and high-resolution Next Generation Sequencing (NGS) method for identification of pathogens directly from clinical samples, and study of the potential application in routine clinical laboratory practice.