NCM 2022: Visualization and analysis of nanopore signal for modification detection and more with Uncalled4
)
Nanopore sequencing works by measuring ionic current as individual DNA or RNA molecules pass through a pore. Many analyses utilize direct alignments of the electrical signal to a nucleotide reference, including detection of nucleotide modifications, polishing, and adaptive sampling. Building off methods developed in Uncalled v1, we present Uncalled4: a toolkit for nanopore signal alignment, analysis, and visualization.