NCM 2022: How to basecall your data and detect methylation
Summary:
Learn how to basecall your nanopore sequencing data and detect methylation — no previous data analysis experience necessary.
Learning objectives:
This masterclass provides an introduction to basecalling and detecting epigenetic modifications in your nanopore sequencing data. In this session, you will learn:
- The different file formats involved in nanopore sequencing data analysis
- How to perform basecalling, without command line experience
- How to directly detect methylation in PCR-free nanopore sequencing data
- How to use real-time targeted sequencing via adaptive sampling for reduced-representation methylation sequencing (RRMS)