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NCM 2022: High-resolution RNA isoform variation analysis by combining the advantages of single-cell RNA and nanopore sequencing


Model Organism: Pigs are one of the ideal models for both human diseases and xenotransplantation due to similar genetic backgrounds and organ sizes. Limitation: However, with the standard single-cell RNA sequencing pipeline, it is only possible to identify the expression level of each gene.

We have established an RNA isoform atlas of pigs to analyse the isoform heterogeneity. We aim to find the preferred dominant isoforms and reveal differential transcript usage (DTU) to detect splicing events at high resolution.

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