NCM 2022: Bringing epigenetics to the Masses: Modbamtools for epigenetic analysis of nanopore data
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Nanopore sequencing has enormous potential in epigenetic applications; unlike traditional sequencing-by-synthesis technologies, it can distinguish covalently modified nucleotides directly through their modulation of the electrolytic current. We can take advantage of the long read lengths (>100kb) generated by nanopore sequencing to precisely call methylation patterns and obtain phased methylation information across the genome. Using exogenous labeling methods, we can measure chromatin accessibility or even protein-DNA interactions.
