NCM 2021: Nanopore sequencing updates using Q20+ and R10.4

This presentation will review improvements in nanopore read length and accuracy over the last year. Protocol improvements have translated to significantly higher throughput using PromethION ultra-long sequencing. Accuracy improvements now permit high-quality SNV calling that exceeds the performance of short reads. Furthermore, the early access Q20+ R10.4 chemistry has potential for improving indel calling as well as achieving phased human genome assemblies with Q40+ accuracy.