NCM 2021: Update from Oxford Nanopore Technologies

00:00 Disclaimer

00:15 Introduction to technology & our goal

01:00 Why nanopore? And accuracy improvements

04:43 How does nanopore sequencing work?

05:47 How can we drive improvements in accuracy and output

08:05 The Nanopore product family

08:35 Accuracy improvements — From the R10.3 to R10.4 nanopores

09:19 PromethION accuracy improvements

09:57 PromethION output (10 Tb of data from a single run)

10:57 Marathon chips: design changes to PromethION Flow Cells

12:41 P2: Low-cost, high-throughput sequencing

13:52 Introduction to Flongle

16:10 Development of our low-cost Flongle Flow Cell

18:05 Can we remove the need for users to flush their flow cell in the future?

19:06 New ASIC and future applications

22:35 Innovation at Oxford Nanopore and how we do it

23:31 Accuracy — the different ways of thinking

24:54 What can affect accuracy?

25:40 A history of nanopore accuracy

27:03 Accuracy — where are we now?

27:38 Duplex accuracy

28:37 Consensus accuracy

29:20 Variant calling accuracy

31:38 Methylation accuracy & our new tool, Remora

33:19 Check out our results with our open data sets

33:51 Application accuracy: barcoding

34:28 Application accuracy: plants

35:40 Application accuracy: short fragments

36:44 Headline accuracy numbers

37:36 MinKNOW

39:15 Basecalling

40:10 Adaptive sampling

41:25 Platform updates

41:45 Platform scalability and accessibility

42:55 Price per Gb

43:31 The versatility and richness of information

46:06 Real, real- time

46:42 Product release process and continuous improvement

49:50 R.10.4 Nanopore and Kit 12 for Q20+ accuracy

50:34 Sample preparation and new kits

51:20 Native barcode kit for Q20+ chemistry

52:03 Rapid kit for Q20+ chemistry

52:35 Multiplex ligation kit

53:25 Ultra-long sequencing kit

54:25 Adaptive sampling

55:15 cDNA PCR sequencing kit

55:14 cDNA PCR sequencing kit: single cell sequencing

57:18 Midnight (ARTIC) sequencing kit

57:49 Sequencing short fragments

58:52 VolTRAX

59:19: Sequencing devices

59:37 MinION

1:00:25 PromethION

1:03:20 P2 Solo

1:03:55 Analysis updates

1:06:04 Tools and workflows

1:07:49 Release timeline