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Nanopore sequencing of single individual Oikopleura dioica

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Why Oikopleura dioica?

Biological features:

  • Global distribution
  • Short life cycle (5 days at 20°C)
  • Chordate body plan
  • Transparent embryonic development
  • Separate sexes

Genetic features:

  • Most compact genome identified in any chordate, only 70Mb
  • >18000 predicted genes
  • Small introns (peak at 47 base pairs, 2.4% >1kb)

Why Nanopore sequencing?

  • Easy library preparation
  • Sequencing in real time
  • Long reads
  • Accessible

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  • Published on: December 12 2019
  • Source: MBSJ 2019

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