Nanopore sequencing for reliable outbreak tracing
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We explored the capabilities of Oxford Nanopore Technology to trace outbreaks in hospitals as an alternative to short-read sequencing. The three year-long outbreak caused by Klebsiella pneumoniae at the University Hospital Leipzig served as a basis for our study. We sequenced 34 random isolates among 114 samples from the outbreak using the SQK-NBD112.24 Kit and compared our findings with existing data from short-read sequencing.