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Nanopore sequencing of African swine fever virus

Publication

Date: 5th November 2019 | Source: Science China Life Sciences

Authors: Lijia Jia, Mengwei Jiang, Ke Wu, Juefu Hu, Yang Wang, Weipeng Quan, Mengchan Hao, Haizhou Liu, Hongping Wei, Wenhui Fan, Wenjun Liu, Rongliang Hu, Depeng Wang, Jing Li, Jianjun Chen, Di Liu.

African swine fever (ASF) is one of the most pathogenic viral diseases in pigs caused by African swine fever virus (ASFV). The fatality rate is almost 100%, which brings huge economic losses to the hog industry in countries with epidemics. China was the first Asian country to have an ASF epidemic, and it spread quickly across the country after the first epidemic was reported in August 2018.

Here, we use the nanopore platform to directly sequence clinical samples from pigs infected with ASFV, and explore the possibility of TGS acquiring large viral genomes such as ASFV.

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