Nanopore direct RNA sequencing of human transcriptomes reveals the complexity of mRNA modifications and crosstalk between regulatory features
Understanding the interactions between various mRNA features remains largely elusive due to the limitations of short-read sequencing technology. Kim and Saville et al. used Oxford Nanopore direct RNA sequencing to simultaneously analyse native mRNA modifications, splicing, and poly-A tail length in leukaemia cells, revealing their complex interactions. This high-resolution view offers new insights into transcript regulation and highlights the therapeutic relevance of targeting RNA modifiers like METTL3 in cancer.
Sample type: human cancer cell lines