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https://vimeo.com/250463272

MinION microbiome profiling: going from on-the-go to go-to?

About https://vimeo.com/250463272

Arwyn Edwards is a microbial ecologist at Aberystwyth University. He is currently a Senior Lecturer in Biology at the Institute of Biological, Environmental & Rural Sciences and Director of the Interdisciplinary Centre for Environmental Microbiology. His research group is focused upon the evolution, structure, function and stability of microbial communities in extreme environments in response to environmental changes at both societally-relevant and “deep” geo/evolutionary timescales. Arwyn particularly enjoys taking Nanopore sequencing to strange new places.

Abstract

Our current perspective of our microbial world is heavily influenced by high throughput DNA sequencing and its limitations. Our interest in MinION sequencing has focused on developing approaches for sequencing in extreme environments to bypass some of the bottlenecks associated with sequencing in microbial ecology. In this talk I will introduce our work in extreme environments as varied as the terrestrial subsurface and Arctic glaciers. Our DeepSeq 1 and 2 experiments were conducted in the Earth’s subsurface and demonstrate the rapid generation of microbiome profiles using lightweight, offline, battery powered metagenomics workflows. Meanwhile, MinION sequencing is becoming integral to our Arctic fieldwork programme, revealing glacier microbial biofilm community structures and metagenomes while deployed in field camps and stations. As the MinION platform, workflows and tools are maturing the prospect of characterising Earth’s microbiomes at source is starting to be realised.

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