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Long-read nanopore sequencing-based draft genome of a Carbapenem-resistant Pseudomonas aeruginosa

Publication

Date: 5th June 2019 | Source: Journal of Global Antimicrobial Resistance

Authors: Xiaoling Yu, Zhaofang Han, Chengsong Ye, Shuyan Zhou, Shaogui Wu, Lifen Han, Ling Zheng, Yiqun Hu, Hanhui Ye.

Objectives
Pseudomonas aeruginosa (P. aeruginosa) is a common Gram-negative bacterium that can cause a series of serious infections, such as lower respiratory tract infection, urinary-tract infections with catheterized patients. Here we reported the draft genome of carbapenem-resistant P. aeruginosa.

Methods
We described the genome of the P. aeruginosa using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads.

Results
The resulting assembly was highly contiguous, containing a total of 6,624,003 bp with a GC content of 66.21%. Annotation identified 6,389 protein-coding genes. Mutations in oprD and MexR gene conferred resistance to carbapenems to the P. aeruginosa isolate.

Conclusions
The draft genome sequence of carbapenem-resistant P. aeruginosa could provide a solid basis for further research on the resistance mechanism and the development of drug therapy for drug resistant genes.

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