Generating assemblies and calling variants

Anthony Doran, Senior Technical Applications Scientist, talked through the approaches available for assembling nanopore sequencing data and calling variants, including single nucleotide variants (SNVs) and structural variants. This involved an overview of the different approaches for assembling nanopore sequence data across a range of experiments – from assembling a human genome to metagenomic assembly – and an introduction to the different file formats involved in nanopore sequencing data analysis and the workflows for calling variants.