Flesh ID: Nanopore sequencing for the identification of meat source
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Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. - In this study, we have established a rapid sequencing protocol to identify animal species within hours. - Sequencing was achieved by nanopore sequencing and data analysis via offline BLAST search. - The whole procedure was conducted in a mobile suitcase lab. As per national and international regulations, the developed assay detected adulteration of pork meat with 0.1 % of Horse, Chicken, Turkey, Cattle, Sheep, Duck, Rabbit, Goat and Donkey. - The developed test could be used on-site as a rapid and mobile detection system to determine contamination of meat products.