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First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (formerly Scedosporium prolificans)

Publication

Date: 29th September 2017 | Source: Genes | Genomes | Genetics

Authors: Ruibang Luo, Aleksey Zimin, Rachael Workman, Yunfan Fan, Geo Pertea, Nina Grossman, Maggie P. Wear, Bei Jia, Heather Miller, Arturo Casadevall, Winston Timp, Sean X. Zhang, Steven L. Salzberg.

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8,896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

Data availability:

This genome project has been deposited at NCBI/GenBank as BioProject PRJNA392827, which includes the raw read data, assembly, and annotation. The assembly is available under accession NLAX00000000; the version described in this paper is version NLAX01000000. The assembly and annotation is also available from the authors' ftp site at ftp.ccb.jhu.edu/pub/data/assembly/L_prolificans.

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