Products & Services
Applications

Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
View all Applications
Resources
News Explore
Contact
Back

Effective downregulation of BCR-ABL tumorigenicity by RNA targeted CRISPR-Cas13a

Publication

Date: 16th November 2019 | Source: BioRxiv

Authors: Aditya Singh, Prateek Bhatia.

CML is a clonal myeloproliferative disorder of pluripotent stem cells driven by a reciprocal translocation between chromosome 9 and 22, forming a BCR-ABL fusion gene. Tyrosine kinase inhibitor drugs like imatinib are mainstay of treatment and cases resistant to these drugs have a poor prognosis in absence of a compatible stem-cell donor. However, with rapid advancements in the gene editing technologies, most studies are now focusing on developing a translational model targeting single gene disorders with prospective permanent cure.

The present study explores the potential application of the RNA targeting CRISPR Cas13a system for effective knockdown of BCR-ABL fusion transcript in K562 cell line.

The CRISPR system was successfully able to lower the expression of downstream genes (pCRKL and pCRK) dependent on the activated BCR-ABL kinase signal by up-to 4.3 folds. The time dependent kinetics also highlighted the significant in vitro suppressive activity to last up to 8 weeks (p-value: 0.025). As per the cDNA sequencing data from Oxford MinION next generation sequencer, the CRISPR activity was 57.45% resulting in 42.55% wild type, 19.15% stop-gain, 10.64% frameshift and 27.66% base substitution transcripts.

These preliminary results highlight an excellent potential application of RNA targeting CRISPRs in Hematological neoplasms like CML and should pave way for further research in this direction.

Read the full text

Recommended for you

Open a chat to talk to our sales team
FAQs

FAQs

Search