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Diagnosis and characterization of canine distemper virus through sequencing by MinION nanopore technology

Publication

Date: 8th February 2019 | Source: Scientific Reports

Authors: Alessia Peserico, Maurilia Marcacci, Daniela Malatesta, Marco Di Domenico, Annamaria Pratelli, Iolanda Mangone, Nicola D’Alterio, Federica Pizzurro, Francesco Cirone, Guendalina Zaccaria, Cesare Cammà, Alessio Lorusso.

Prompt identification of the causative pathogen of an infectious disease is essential for the choice of treatment or preventive measures. In this perspective, nucleic acids purified from the brain tissue of a dog succumbed after severe neurological signs were processed with the MinION (Oxford Nanopore Technologies, Oxford UK) sequencing technology. Canine distemper virus (CDV) sequence reads were detected. Subsequently, a specific molecular test and immunohistochemistry were used to confirm the presence of CDV RNA and antigen, respectively, in tissues. This study supports the use of the NGS in veterinary clinical practice with potential advantages in terms of rapidity and broad-range of molecular diagnosis.

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