Deep transcriptomic sampling with long-read single cell RNA sequencing
About Mike Clark
Mike Clark is head of the Transcriptomics and Neurogenetics group at the University of Melbourne in Australia. His research sits at the intersection of genomics and neuroscience, utilizing a number of genomic approaches, including nanopore sequencing, to investigate gene expression and function in the human brain and in neuropsychiatric disorders.
Single cell RNA-seq (scRNA-seq) is rapidly gaining favour for understanding biology. Long-read methods have great potential for scRNA-seq by facilitating the identification and quantification of gene isoforms, allowing cell specific variation in isoform expression and splicing to be characterised. Previous methods for transcriptome-wide long-read scRNA-seq have been limited by low numbers of cells and/or few reads per cell. We have developed an improved nanopore long-read scRNA-seq method that allows the profiling of hundreds of single-cells at high read-depth and demonstrate its use by profiling five human cancer cell lines. These results demonstrate the power of long-read sequencing to characterise gene and isoform expression in single cells.