Dancing the Nanopore Limbo: Low DNA input for successful microbial metagenomics with Oxford Nanopore Technologies
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Short-read assemblies often result in incomplete and/or highly fragmented metagenome-assembled genomes (MAGs)
• Nanopore long reads greatly improve the assembly quality of MAGs
• Recommended DNA quantity usually exceeds the recoverable amount of DNA isolated from environmental samples
⇒ Evaluation of lower-than-recommended DNA quantities for Nanopore library preparation by determining sequencing quality, community composition, assembly quality and recovery of MAG
