Concerted modification of nucleotides at functional centers of the ribosome revealed by single-molecule RNA modification profiling

A method enabling single-molecule profiling of RNA modifications is developed and reveals heterogeneous classes of modified ribosomes.

  • Most rRNA 2´O methylation and pseudouridylation modifications are independent of each other.
  • Nucleotides in functional centers of the ribosome are modified in a concerted fashion.
  • Loss of function for RNA helicases Dbp3 and Prp43 produce discrete overlapping subpopulations of incompletely modified ribosomes.
  • RNA modification profiles are resilient to rapidly changing nutrient conditions and perturbation of translation

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