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Clive Brown

Clive Brown: NCM update 2021

At the Nanopore Community Meeting, Clive Brown gives an update on the latest developments in technology at Oxford Nanopore.

00:00 Disclaimer

00:05 Introduction

01:00 Our goal

01:20 How nanopore sequencing works

06:44 Read length == Fragment length

09:25 Short fragment mode

12:40 Accuracy — Recap

14:48 Q20+ raw read accuracy

18:01 Accuracy — Where are we now?

21:50 Accuracy — Continuous improvement

25:05 Duplex — Reading both strands

31:01 Accuracy — Methylation

37:09 Accuracy — Enzyme kinetics

38:15 Accuracy — R10.4 and longer nanopores

39:44 The relationship between accuracy, speed and temperature

41:50 Chemistries — Movement schemes

45:26 Chemistries — Outy sequencing

54:02 Chemistries — Outy adaptive accuracy, highly accurate sequencing of rare single-molecules

56:57 Liquid biopsy workflow

59:04 Skunkworx: liquid biopsy devices

1:02:30 P2

1:03:50 P2 solo

1:04:47 Browser calling: web based basecalling

1:07:33 MinION Mk1D

1:10:50 Accuracy headlines

1:12:05 Org.one

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