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An assessment of Oxford Nanopore sequencing for human gut metagenome profiling: a pilot study of head and neck cancer patients


Gut metagenome profiling using the Oxford Nanopore Technology (ONT) sequencer was assessed in a pilot-sized study of 10 subjects. The taxonomic abundance of gut microbiota derived from ONT was comparable with Illumina Technology (IT) for the high-abundance species. IT better detected low-abundance species through amplification, when material was limited.

Authors: Thidathip Wongsurawat, Mayumi Nakagawa, Omar Atiq, Hannah N. Coleman, Piroon Jenjaroenpun, James I. Allred, Angela Trammel, Pantakan Puengrang, David W. Ussery, Intawat Nookaew

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