A successor to Sanger: accurate, full-length, and haplotype-resolved amplicon sequencing with Oxford Nanopore

Oxford Nanopore sequencing enables rapid, high-throughput amplicon analysis with Sanger-level accuracy, generating consensus sequences in as little as 30 minutes, even in highly multiplexed runs. By sequencing full-length, multi-kilobase amplicons in single reads, nanopore technology preserves variant linkage and resolves haplotypes across entire targets — accessing an additional layer of biological insight that is not accessible using Sanger sequencing alone.

In this application note, we evaluate Oxford Nanopore sequencing for both routine and complex amplicon analysis using matched datasets generated with both Oxford Nanopore and Sanger sequencing.

In this application note, you will:

• Learn how nanopore sequencing enables rapid, same-day amplicon consensus generation at scale
• Discover the advantages of full-length, haplotype-resolved analysis for accurate variant detection
• Explore benchmarking data that validates performance across diverse sample types