Amplicon sequencing of pasteurised retail dairy enables genomic surveillance of H5N1 avian influenza virus in United States cattle

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Lail et al. used tiled-amplicon Oxford Nanopore sequencing to detect and characterise H5N1 virus in pasteurised dairy, achieving over 90% genome coverage in viral RNA-positive samples. This method offers a rapid, accessible surveillance solution in regions without on-farm testing, supporting early detection and response to viral spread.

Read more on viral amplicon sequencing with Oxford Nanopore technology

Sample type: retail dairy products

Kit: Native Barcoding Kit

Authors: Andrew J. Lail, William C. Vuyk, Heather Machkovech, Nicholas R. Minor, Nura R. Hassan, Rhea Dalvie, Isla E. Emmen, Sydney Wolf, Annabelle Kalweit, Nancy Wilson, Christina M. Newman, Patrick Barros Tiburcio, Andrea Weiler, Thomas C. Friedrich, David H. O’Connor
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PubMed
  • Published on: June 13 2025
  • Source: PLOS One