Characterising structural variants in FFPE cancer research samples
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Overview
Structural variation is a key mutational process in cancer and can impact whole genes or chromosomes. Formalin-fixed, paraffin-embedded (FFPE) sampling is a widely used, simple method to preserve important cancer research samples, but it cases DNA damage.
This end-to-end nanopore sequencing workflow provides a simple method to extract and sequence DNA spanning several kilobases from FFPE samples for accurate structural variant (SV) detection and analysis.
In this workflow overview, you will:
- Find out how unrestricted nanopore read lengths enhances SV characterisation in FFPE cancer research samples
- Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
- Learn about our recommended sequencing kit and devices