Characterising influenza type A and B virus genomes through whole-genome nanopore sequencing

Overview

Influenza is a global health concern due to annual epidemics of the disease and its potential to cause a pandemic because of novel strain variants. Whole-genome sequencing enables ongoing surveillance and identification of new emergent strains and can therefore provide information to inform the development of seasonal vaccines.

This overview describes how to generate accurate whole-genome sequences of influenza A and B viruses by PCR amplification and multiplexed nanopore sequencing.

In this protocol overview, you will:

• Learn the key steps in the sequencing protocol
• Discover our sequencing workflow , starting from the recommended extraction method, through to primary analysis with EPI2ME
• Find out our recommended sequencing kit and devices