Plant research

Unrestricted read length (>4 Mb achieved)

• Resolve complex and repetitive genomic regions such as structural variants and transposons
• Generate high-quality de novo plant genomes and correct reference genomes
• Analyse long-range haplotypes and phasing, and get greater insight into polyploid genomes
• Accurately annotate plant genomes using full-length transcripts
• Get isoform-level transcriptome characterisation and quantification
• End-to-end sequencing of structural variants

Read length typically 50–300 bp

Short reads do not typically span entire regions of interest, including repeats and structural variants, or full-length RNA transcripts, resulting in fragmented assemblies and ambiguous transcript isoform identification.

Direct, amplification-free protocols

• Detect base modifications (e.g. methylation) as standard — no additional prep required
• Eliminate amplification bias, GC-bias, and read length limitations
• Expand the utility of targeted sequencing with long, amplification-free reads using the Cas9 Sequencing Kit or real-time, on-device adaptive sampling

Amplification required

Amplification can introduce bias — reducing uniformity of coverage with the potential for coverage gaps — and removes base modifications, necessitating additional sample prep, sequencing runs, and expense.

Flexible and on-demand

• Scale to your throughput needs
• Sequence in the lab or field with portable Flongle and MinION
• Tackle large plant genome projects with flexible, high-throughput GridION and PromethION devices
• No sample batching required

Limited flexibility

Platform costs and infrastructure requirements can limit global accessibility. Sample batching may also be required for optimal efficiency, potentially delaying results.

Streamlined workflows

• Prepare DNA samples for sequencing in as little as 10 minutes, including multiplexing
• Use whole genome, metagenomic, targeted, and RNA sequencing approaches
• Automate sample prep using the portable VolTRAX device

Laborious workflows

Typically, lengthy sample preparation requirements and long sequencing run times, reducing workflow efficiency.

Real-time data streaming

• Get immediate access to results for time critical applications such as plant pathogen identification
• Enrich targeted regions based on real-time sequence composition using adaptive sampling
• Stop sequencing when sufficient data generated — wash and reuse flow cell
• Use simple EPI2ME workflows for real-time microbiome and plant pathogen analysis

Fixed run time with bulk data delivery

Increased time-to-result and inability to identify workflow errors until it’s too late, plus additional complexities of handing large volumes of bulk data.