07 February 2024
- Updated Flow cell wash method in line with updated Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) protocol.
- Updated links in protocol
- Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between.
- Updated method with more extensive instructions on use and handling.
17th July 2023
- Updated recommendations for running a second library on a PromethION Flow Cell straigh away and added a step to remove waste after 1 hour incubation during nuclease wash.
March 2023
- Updated the protocol title from 'Running multiple PromethION flow cells' to 'Loading multiple PromethION Flow Cells'
- Updated the introduction to the protocol
- Updated the equipment and consumables for Kit 14 chemistry
- Updated the flow cell loading animations and included a new video illustrating the entire new process
- Included excess volumes required for scaling up priming mix volumes
- Updated the screenshot of what users can expect to see on MinKNOW when washing and reusing a flow cells
March 2023
- Updated the protocol title from 'Running multiple PromethION flow cells' to 'Loading multiple PromethION Flow Cells'
- Updated the introduction to the protocol
- Updated the equipment and consumables for Kit 14 chemistry
- Updated the flow cell loading animations and included a new video illustrating the entire new process
- Included excess volumes required for scaling up priming mix volumes
- Updated the screenshot of what users can expect to see on MinKNOW when washing and reusing a flow cells
22nd July 2021
In the "Washing multiple flow cells on a PromethION" section, the instructions for washing the flow cells have been updated with the correct volumes of Wash Mix and Wash Diluent:
- For __24 flow cells:__ Add __48 µl__ of thawed and mixed Wash Mix (WMX) to __9.552 ml__ of thawed and mixed Wash Diluent (DIL)
- For __48 flow cells:__ Add __96 µl__ of thawed and mixed Wash Mix (WMX) to __19.104 ml__ of thawed and mixed Wash Diluent (DIL)
10th June 2021
In the "Priming and loading multiple flow cells on a PromethION" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins