29th January 2025
In "To run a second library on multiple PromethION Flow Cells straight away", a new tip has been added: "Use a new pipette tip for every step when priming washed flow cells that have previously been used for sequencing, to avoid cross-contamination"
2/4/2025
PFC_9097_v1_revN_29Jan2025
English
PromethION
29th January 2025
In "To run a second library on multiple PromethION Flow Cells straight away", a new tip has been added: "Use a new pipette tip for every step when priming washed flow cells that have previously been used for sequencing, to avoid cross-contamination"
2/7/2024
PFC_9097_v1_revM_18Mar2020
English
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07 February 2024
- Updated Flow cell wash method in line with updated Flow Cell Wash Kit (EXP-WSH004 or EXP-WSH004-XL) protocol.
- Updated links in protocol
- Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between.
- Updated method with more extensive instructions on use and handling.
17th July 2023
- Updated recommendations for running a second library on a PromethION Flow Cell straigh away and added a step to remove waste after 1 hour incubation during nuclease wash.
March 2023
- Updated the protocol title from 'Running multiple PromethION flow cells' to 'Loading multiple PromethION Flow Cells'
- Updated the introduction to the protocol
- Updated the equipment and consumables for Kit 14 chemistry
- Updated the flow cell loading animations and included a new video illustrating the entire new process
- Included excess volumes required for scaling up priming mix volumes
- Updated the screenshot of what users can expect to see on MinKNOW when washing and reusing a flow cells
March 2023
- Updated the protocol title from 'Running multiple PromethION flow cells' to 'Loading multiple PromethION Flow Cells'
- Updated the introduction to the protocol
- Updated the equipment and consumables for Kit 14 chemistry
- Updated the flow cell loading animations and included a new video illustrating the entire new process
- Included excess volumes required for scaling up priming mix volumes
- Updated the screenshot of what users can expect to see on MinKNOW when washing and reusing a flow cells
22nd July 2021
In the "Washing multiple flow cells on a PromethION" section, the instructions for washing the flow cells have been updated with the correct volumes of Wash Mix and Wash Diluent:
- For __24 flow cells:__ Add __48 µl__ of thawed and mixed Wash Mix (WMX) to __9.552 ml__ of thawed and mixed Wash Diluent (DIL)
- For __48 flow cells:__ Add __96 µl__ of thawed and mixed Wash Mix (WMX) to __19.104 ml__ of thawed and mixed Wash Diluent (DIL)
10th June 2021
In the "Priming and loading multiple flow cells on a PromethION" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
ONT plc is a member of the producer compliance scheme run by ERP UK Ltd, who manage the submission of documentation in support of WEEE compliance for ONT plc’s manufacture and supply of Electrical and Electronic equipment in the UK.
