January 2023
- Updated Flongle Flow Cell Priming Kit name to Flongle Sequencing Expansion (EXP-FSE001)
- Updated the "Priming and loading the SpotON flow cell" for Min/Grid as it had incorrect information referring to kit 12 chemistry
- Updated the "Priming and loading the flow cell" for Prom to new standard
January 2023
- Updated Flongle Flow Cell Priming Kit name to Flongle Sequencing Expansion (EXP-FSE001)
- Updated the "Priming and loading the SpotON flow cell" for Min/Grid as it had incorrect information referring to kit 12 chemistry
- Updated the "Priming and loading the flow cell" for Prom to new standard
January 2023
- Updated Flongle Flow Cell Priming Kit name to Flongle Sequencing Expansion (EXP-FSE001)
- Updated the "Priming and loading the SpotON flow cell" for Min/Grid as it had incorrect information referring to kit 12 chemistry
- Updated the "Priming and loading the flow cell" for Prom to new standard
January 2023
- Updated Flongle Flow Cell Priming Kit name to Flongle Sequencing Expansion (EXP-FSE001)
- Updated the "Priming and loading the SpotON flow cell" for Min/Grid as it had incorrect information referring to kit 12 chemistry
- Updated the "Priming and loading the flow cell" for Prom to new standard
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 6 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
22nd December 2021
In the Flongle branch of the protocol, "Equipment and consumables", added a note to the Flongle Sequencing Expansion information: "Oxford Nanopore Technologies deem the useful life of the Flow Cell Expansion to be 3 months from receipt by the customer."
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed reference to RAP Top Up expansion not currently available on the store
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed reference to RAP Top Up expansion not currently available on the store
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed reference to RAP Top Up expansion not currently available on the store
6th December 2021
- Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
- Removed reference to RAP Top Up expansion not currently available on the store
7th October 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
7th October 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
7th October 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
7th October 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A clarifying image has been added under "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
23rd September 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
- Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
23rd September 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
- Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
23rd September 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
- Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
23rd September 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell":
- A note has been added: "The adapter needs to be plugged into your device, and the device should be plugged in and powered on before inserting the Flongle flow cell."
- Flow cell loading instructions have been changed such that a 200 μl pipette is used to load the sequencing library into the flow cell.
24th June 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
24th June 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
24th June 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
24th June 2021
In the Flongle branch of the protocol, "Loading the Flongle flow cell" step, an amendment has been made to step 8: "Loading Beads II (LBII) mixed immediately before use, or Loading Solution (LS), if using. LS can be used instead of LBII when preparing libraries with the Ligation Sequencing Kit (SQK-LSK110)"
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
21st April 2021
In the Flongle branch of the protocol:
- "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic
- "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl."
- "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."
21st April 2021
In the Flongle branch of the protocol:
- "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic
- "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl."
- "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."
21st April 2021
In the Flongle branch of the protocol:
- "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic
- "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl."
- "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."
21st April 2021
In the Flongle branch of the protocol:
- "Equipment and consumables", "Library preparation" and "Loading the Flongle flow cell" steps: added additional information about flow cell deterioration/saturation, and updated the Flongle Sequencing Expansion information to mention Loading Solution (LS) and reduction of flow cell deterioration when storing components in glass vials instead of plastic
- "Library preparation" step: updated the loading recommendations to "Following standard input recommendations, the protocol should produce enough final library (adapted DNA in EB) to load at least two Flongle flow cells. We recommend reserving enough library to load a second Flongle flow cell. We recommend loading 3-20 fmol of this final prepared library onto the flow cell. Loading more than 50 fmol can have a detrimental effect on throughput. Dilute the library in EB or nuclease free water up to a final volume of 5 μl."
- "Loading the Flongle flow cell" step: added the warning "ALWAYS wear gloves when handling Flongle flow cells and adapters to avoid damage to the flow cell or adapter."