29th June 2023
- Moved and reformatted the required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling".
- Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
9/7/2023
PCTR_9125_v110_revI_24Mar2021
English
MinION
29th June 2023
- Moved and reformatted the required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling".
- Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
4th October 2021
- Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section
4th October 2021
- Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section
20th July 2021
- In 'Addition of rapid barcodes', steps have been re-arranged to make instructions clearer. Users are now instructed to aliquot water into a clean 96-well plate (Barcode Attachment Plate) first, before pooling PCR pools and adding to the Barcode Attachment Plate. Finally, the barcodes are transferred to the Barcode Attachment Plate.
- Updated steps 'PCR' and 'Pooling samples and clean-up' to include information for volume per sample.
- Updated images in steps 'Reverse transcription', 'PCR' and 'Addition of rapid barcodes' with clearer images of the plates.
20th July 2021
- In 'Addition of rapid barcodes', steps have been re-arranged to make instructions clearer. Users are now instructed to aliquot water into a clean 96-well plate (Barcode Attachment Plate) first, before pooling PCR pools and adding to the Barcode Attachment Plate. Finally, the barcodes are transferred to the Barcode Attachment Plate.
- Updated steps 'PCR' and 'Pooling samples and clean-up' to include information for volume per sample.
- Updated images in steps 'Reverse transcription', 'PCR' and 'Addition of rapid barcodes' with clearer images of the plates.
16th June 2021
- Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
16th June 2021
- Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.