PCR tiling of SARS-CoV-2 virus - rapid barcoding and Midnight RT PCR Expansion (SQK-RBK110.96 and EXP-MRT001)

DateVersionLanguageDeviceChanges
6/29/2023MRT_9127_v110_revW_14Jul2021EnglishGridION
29th June 2023 - Moved required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling". - Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
6/29/2023MRT_9127_v110_revW_14Jul2021EnglishMinION
29th June 2023 - Moved required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling". - Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
6/29/2023MRT_9127_v110_revW_14Jul2021EnglishPromethION
29th June 2023 - Moved required MinKNOW settings from the "Priming and loading a flow cell" sections into "Data acquisition and basecalling". - Ensured MinKNOW barcode settings are consistent with our recommendations in the "downstream analysis" section.
May 3rd, 2023MRT_9127_v110_revV_14Jul2021EnglishPromethion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
May 3rd, 2023MRT_9127_v110_revV_14Jul2021EnglishGridion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
May 3rd, 2023MRT_9127_v110_revV_14Jul2021EnglishMinion Download 3rd May - Updated the Midnight Primer sequences to include the V3 primer spike-ins
March 15th, 2023MRT_9127_v110_revU_14Jul2021EnglishPromethion Download February 2023 - Removed the checklist download to fix formatting issues.
March 15th, 2023MRT_9127_v110_revU_14Jul2021EnglishGridion Download February 2023 - Removed the checklist download to fix formatting issues.
March 15th, 2023MRT_9127_v110_revU_14Jul2021EnglishMinion Download February 2023 - Removed the checklist download to fix formatting issues.
January 11th, 2023MRT_9127_v110_revT_14Jul2021EnglishPromethion Download 11th January 2023 - Included the "Required settings in MinKNOW" to updated the PromethION "Priming and loading the flow cell" section.
January 11th, 2023MRT_9127_v110_revT_14Jul2021EnglishGridion Download 11th January 2023 - Included the "Required settings in MinKNOW" to updated the PromethION "Priming and loading the flow cell" section.
January 11th, 2023MRT_9127_v110_revT_14Jul2021EnglishMinion Download 11th January 2023 - Included the "Required settings in MinKNOW" to updated the PromethION "Priming and loading the flow cell" section.
January 11th, 2023MRT_9127_v110_revS_14Jul2021EnglishPromethion Download 11th January 2023 - Included the "Required settings in MinKNOW" to updated the PromethION "Priming and loading the flow cell" section.
January 11th, 2023MRT_9127_v110_revS_14Jul2021EnglishGridion Download 11th January 2023 - Included the "Required settings in MinKNOW" to updated the PromethION "Priming and loading the flow cell" section.
January 11th, 2023MRT_9127_v110_revS_14Jul2021EnglishMinion Download 11th January 2023 - Included the "Required settings in MinKNOW" to updated the PromethION "Priming and loading the flow cell" section.
January 6th, 2023MRT_9127_v110_revR_14Jul2021EnglishPromethion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
January 6th, 2023MRT_9127_v110_revR_14Jul2021EnglishGridion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
January 6th, 2023MRT_9127_v110_revR_14Jul2021EnglishMinion Download January 2023 - Changed the PromethION "Priming and loading the flow cell" section to the updated version.
August 10th, 2022MRT_9127_v110_revQ_14Jul2021EnglishPromethion Download 21th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022MRT_9127_v110_revQ_14Jul2021EnglishGridion Download 21th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022MRT_9127_v110_revQ_14Jul2021EnglishMinion Download 21th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 13th, 2022MRT_9127_v110_revP_14Jul2021EnglishPromethion Download 14th April 2022 Protocol name change
April 13th, 2022MRT_9127_v110_revP_14Jul2021EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022MRT_9127_v110_revP_14Jul2021EnglishMinion Download 14th April 2022 Protocol name change
February 18th, 2022MRT_9127_v110_revO_14Jul2021EnglishPromethion Download 18th February 2022 - Minor correction to "Pooling samples and clean-up" for the PromethION branch
February 18th, 2022MRT_9127_v110_revO_14Jul2021EnglishGridion Download 18th February 2022 - Minor correction to "Pooling samples and clean-up" for the PromethION branch
February 18th, 2022MRT_9127_v110_revO_14Jul2021EnglishMinion Download 18th February 2022 - Minor correction to "Pooling samples and clean-up" for the PromethION branch
February 2nd, 2022MRT_9127_v110_revN_14Jul2021EnglishPromethion Download 2nd February 2022 - Updated Midnight Primer Pool B to include the additional primer
February 2nd, 2022MRT_9127_v110_revN_14Jul2021EnglishGridion Download 2nd February 2022 - Updated Midnight Primer Pool B to include the additional primer
February 2nd, 2022MRT_9127_v110_revN_14Jul2021EnglishMinion Download 2nd February 2022 - Updated Midnight Primer Pool B to include the additional primer
December 17th, 2021MRT_9127_v110_revM_14Jul2021EnglishPromethion Download 17th Dec 2021 - Updated the PCR cycle conditions
December 17th, 2021MRT_9127_v110_revM_14Jul2021EnglishGridion Download 17th Dec 2021 - Updated the PCR cycle conditions
December 17th, 2021MRT_9127_v110_revM_14Jul2021EnglishMinion Download 17th Dec 2021 - Updated the PCR cycle conditions
December 6th, 2021MRT_9127_v110_revL_14Jul2021EnglishPromethion Download 6th December 2021 - Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP) - Removed snippet for MinKNOW settings in " Sequencing and data analysis: Data acquisition and basecalling" as the required settings for Midnight are default when the Midnight expansion is selected in 'Kit selection' when setting up a MinKNOW run
December 6th, 2021MRT_9127_v110_revL_14Jul2021EnglishGridion Download 6th December 2021 - Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP) - Removed snippet for MinKNOW settings in " Sequencing and data analysis: Data acquisition and basecalling" as the required settings for Midnight are default when the Midnight expansion is selected in 'Kit selection' when setting up a MinKNOW run
December 6th, 2021MRT_9127_v110_revL_14Jul2021EnglishMinion Download 6th December 2021 - Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP) - Removed snippet for MinKNOW settings in " Sequencing and data analysis: Data acquisition and basecalling" as the required settings for Midnight are default when the Midnight expansion is selected in 'Kit selection' when setting up a MinKNOW run
December 6th, 2021MRT_9127_v110_revK_14Jul2021EnglishPromethion Download 6th December 2021 - Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
December 6th, 2021MRT_9127_v110_revK_14Jul2021EnglishGridion Download 6th December 2021 - Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
December 6th, 2021MRT_9127_v110_revK_14Jul2021EnglishMinion Download 6th December 2021 - Updated kit contents image with the updated vial label and name from SPRI to AMPure XP Beads (AXP)
November 26th, 2021MRT_9127_v110_revJ_14Jul2021EnglishPromethion Download 23rd November 2021 - Updated the 'Data acquisition and basecalling' section to include information for starting an automatic Midnight analysis run on GridION devices.
November 26th, 2021MRT_9127_v110_revJ_14Jul2021EnglishGridion Download 23rd November 2021 - Updated the 'Data acquisition and basecalling' section to include information for starting an automatic Midnight analysis run on GridION devices.
November 26th, 2021MRT_9127_v110_revJ_14Jul2021EnglishMinion Download 23rd November 2021 - Updated the 'Data acquisition and basecalling' section to include information for starting an automatic Midnight analysis run on GridION devices.
October 20th, 2021MRT_9127_v110_revH_14Jul2021EnglishPromethion Download 4th October 2021 - Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section - Updated the PCR mastermix ratios as well as reducing the volume of RT product taken forward
October 20th, 2021MRT_9127_v110_revH_14Jul2021EnglishGridion Download 4th October 2021 - Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section - Updated the PCR mastermix ratios as well as reducing the volume of RT product taken forward
October 20th, 2021MRT_9127_v110_revH_14Jul2021EnglishMinion Download 4th October 2021 - Included information outlining the differences between this protocol and another similar protocol in the 'Overview of the protocol' section - Updated the PCR mastermix ratios as well as reducing the volume of RT product taken forward
September 9th, 2021MRT_9127_v110_revG_14Jul2021EnglishPromethion Download 9th Sept 2021 - Updated equipment and consumables to use a Qubit fluorometer reader rather than a plate reader. - Qubit dsDNA BR Assay Kit has been changed to Qubit dsDNA HS Assay Kit in the equipment and consumables section and pooling and clean-up step
September 9th, 2021MRT_9127_v110_revG_14Jul2021EnglishGridion Download 9th Sept 2021 - Updated equipment and consumables to use a Qubit fluorometer reader rather than a plate reader. - Qubit dsDNA BR Assay Kit has been changed to Qubit dsDNA HS Assay Kit in the equipment and consumables section and pooling and clean-up step
September 9th, 2021MRT_9127_v110_revG_14Jul2021EnglishMinion Download 9th Sept 2021 - Updated equipment and consumables to use a Qubit fluorometer reader rather than a plate reader. - Qubit dsDNA BR Assay Kit has been changed to Qubit dsDNA HS Assay Kit in the equipment and consumables section and pooling and clean-up step
August 31st, 2021MRT_9127_v110_revF_14Jul2021EnglishPromethion Download 31st August 2021 - Updated step: Pooling samples and clean-up in the PromethION branch so that the DNA library is made up to 23 µl with Elution Buffer (EB), rather than 11 µl as per for MinION flow cells.
August 31st, 2021MRT_9127_v110_revF_14Jul2021EnglishGridion Download 31st August 2021 - Updated step: Pooling samples and clean-up in the PromethION branch so that the DNA library is made up to 23 µl with Elution Buffer (EB), rather than 11 µl as per for MinION flow cells.
August 31st, 2021MRT_9127_v110_revF_14Jul2021EnglishMinion Download 31st August 2021 - Updated step: Pooling samples and clean-up in the PromethION branch so that the DNA library is made up to 23 µl with Elution Buffer (EB), rather than 11 µl as per for MinION flow cells.
August 18th, 2021MRT_9127_v110_revE_14Jul2021EnglishPromethion Download 16th July 2021 - Updated to include instructions for loading on PromethION flow cells
August 18th, 2021MRT_9127_v110_revE_14Jul2021EnglishGridion Download 16th July 2021 - Updated to include instructions for loading on PromethION flow cells
August 18th, 2021MRT_9127_v110_revE_14Jul2021EnglishMinion Download 16th July 2021 - Updated to include instructions for loading on PromethION flow cells
August 16th, 2021MRT_9127_v110_revD_14Jul2021EnglishGridion Download 16th July 2021 - Introduction updated to remove reference to amplicon overlap
August 16th, 2021MRT_9127_v110_revD_14Jul2021EnglishMinion Download 16th July 2021 - Introduction updated to remove reference to amplicon overlap
August 16th, 2021MRT_9127_v110_revC_14Jul2021EnglishGridion Download 16th July 2021 - Introduction updated to remove reference to amplicon overlap
August 16th, 2021MRT_9127_v110_revC_14Jul2021EnglishMinion Download 16th July 2021 - Introduction updated to remove reference to amplicon overlap
July 20th, 2021MRT_9127_v110_revB_14Jul2021EnglishGridion Download 20th July 2021 - Updated images in sections: 'Reverse transcription', 'PCR', and 'Addition of rapid barcodes' to clearly show the plates.
July 20th, 2021MRT_9127_v110_revB_14Jul2021EnglishMinion Download 20th July 2021 - Updated images in sections: 'Reverse transcription', 'PCR', and 'Addition of rapid barcodes' to clearly show the plates.
July 14th, 2021MRT_9127_v110_revA_14Jul2021EnglishGridion Download -
July 14th, 2021MRT_9127_v110_revA_14Jul2021EnglishMinion Download -