Ligation sequencing gDNA - low input by PCR (SQK-LSK109 with EXP-PCA001) (LWP_9087_v109_revO_14Aug2019)

DateVersionLanguageDeviceChanges
3/10/2023LWP_9087_v109_revO_14Aug2019EnglishGridION
February 2023 - Updated product phase to 'Legacy'
3/10/2023LWP_9087_v109_revO_14Aug2019EnglishMinION
February 2023 - Updated product phase to 'Legacy'
August 10th, 2022LWP_9087_v109_revN_14Aug2019EnglishGridion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
August 10th, 2022LWP_9087_v109_revN_14Aug2019EnglishMinion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 6th, 2022LWP_9087_v109_revN_06Jul2022EnglishGridion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 6th, 2022LWP_9087_v109_revN_06Jul2022EnglishMinion Download 06th July 2022 - Updated the snippet: "Prepare the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair / dA-tailing Module reagents in accordance with manufacturer’s instructions, and place on ice." to also include the manufacturers recommendations.
July 4th, 2022LWP_9087_v109_revM_14Aug2019EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022LWP_9087_v109_revM_14Aug2019EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 14th, 2022LWP_9087_v109_revL_14Aug2019EnglishGridion Download 14th April 2022 Protocol name change
April 14th, 2022LWP_9087_v109_revL_14Aug2019EnglishMinion Download 14th April 2022 Protocol name change
April 14th, 2022LWP_9087_v109_revL_14Aug2019EnglishAny Download 14th April 2022 Protocol name change
January 28th, 2022LWP_9087_v109_revK_14Aug2019EnglishGridion Download 28th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
January 28th, 2022LWP_9087_v109_revK_14Aug2019EnglishMinion Download 28th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep In "Priming and loading the flow cell", further tips/notes have been added regarding preparing the library and flow cell loading.
September 23rd, 2021LWP_9087_v109_revJ_14Aug2019EnglishGridion Download 23rd Sept 2021 - Updated compatibilities snippet in the 'Overview of the protocol' and updated the flow cell loading recommendations
September 23rd, 2021LWP_9087_v109_revJ_14Aug2019EnglishMinion Download 23rd Sept 2021 - Updated compatibilities snippet in the 'Overview of the protocol' and updated the flow cell loading recommendations
September 13th, 2021LWP_9087_v109_revI_14Aug2019EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021LWP_9087_v109_revI_14Aug2019EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
January 20th, 2021LWP_9087_v109_revH_14Aug2019EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021LWP_9087_v109_revH_14Aug2019EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
February 21st, 2020LWP_9087_v109_revG_14Aug2019EnglishGridion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
February 21st, 2020LWP_9087_v109_revG_14Aug2019EnglishMinion Download 21st Feb 2020 Added 'Library storage recommendations' snippet before flow cell loading step
February 4th, 2020LWP_9087_v109_revF_14Aug2019EnglishGridion Download 30th September 2019 The optional DNA fragmentation step has been removed from the protocol, since it is now available as a [stand-alone protocol](https://community.nanoporetech.com/extraction_methods/dna-fragmentation).
February 4th, 2020LWP_9087_v109_revF_14Aug2019EnglishMinion Download 30th September 2019 The optional DNA fragmentation step has been removed from the protocol, since it is now available as a [stand-alone protocol](https://community.nanoporetech.com/extraction_methods/dna-fragmentation).
September 27th, 2019LWP_9087_v109_revE_14Aug2019EnglishGridion Download 27th September 2019 Updated and expanded Troubleshooting guide
September 27th, 2019LWP_9087_v109_revE_14Aug2019EnglishMinion Download 27th September 2019 Updated and expanded Troubleshooting guide
September 20th, 2019LWP_9087_v109_revD_14Aug2019EnglishGridion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
September 20th, 2019LWP_9087_v109_revD_14Aug2019EnglishMinion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019LWP_9087_v109_revC_14Aug2019EnglishGridion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019LWP_9087_v109_revC_14Aug2019EnglishMinion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 14th, 2019LWP_9087_v109_revB_14Aug2019EnglishGridion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019LWP_9087_v109_revB_14Aug2019EnglishMinion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
July 19th, 2019LWP_9087_v109_revA_19Jul2019English- Download -