30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
ACDE_9163_v114_revW_30Jan2025
English
GridION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
ACDE_9163_v114_revW_30Jan2025
English
MinION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
ACDE_9163_v114_revW_30Jan2025
English
PromethION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024
ACDE_9163_v114_revV_12Dec2024
English
Flongle
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
ACDE_9163_v114_revV_12Dec2024
English
GridION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
ACDE_9163_v114_revV_12Dec2024
English
MinION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
ACDE_9163_v114_revV_12Dec2024
English
PromethION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024
ACDE_9163_v114_revU_29Jun2022
English
Flongle
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
ACDE_9163_v114_revU_29Jun2022
English
GridION
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
ACDE_9163_v114_revU_29Jun2022
English
MinION
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
ACDE_9163_v114_revU_29Jun2022
English
PromethION
21st August 2024
- Updated sample input recommendations with table for different DNA size.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
30th July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
30th July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
30th July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
08 Aug 2024
Added sentence to give LMD fragment length in Introduction to the protocol/Equipment and consumables/Control Expansion (EXP-CTL001) contents
"Lambda DNA (LMD) fragment length is 48.5 kb."
30th July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
19th August 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
- Update the equipment and consumables section to only be relevant for amplicon DNA
- Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
19th August 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
- Update the equipment and consumables section to only be relevant for amplicon DNA
- Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
19th August 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
- Update the equipment and consumables section to only be relevant for amplicon DNA
- Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
19th August 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
- Update the equipment and consumables section to only be relevant for amplicon DNA
- Removed references to duplex basecalling and Guppy in the "Sequencing and data analysis" section.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
- Updated protocol to use 80% ethanol rather than 70% ethanol.
27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
- Updated protocol to use 80% ethanol rather than 70% ethanol.
27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
- Updated protocol to use 80% ethanol rather than 70% ethanol.
27th October 2022
- Renamed "DNA repair and end-prep step" to "End-prep" and updated step to not use FFPE repair mix as DNA repair is not needed for amplicon input.
- Updated protocol to use 80% ethanol rather than 70% ethanol.