Ligation sequencing amplicons - native barcoding (SQK-LSK109 with EXP-NBD196) (NBA_9102_v109_revO_09Jul2020)

DateVersionLanguageDeviceChanges
3/10/2023NBA_9102_v109_revO_09Jul2020EnglishGridION
February 2023 - Updated product phase to 'Legacy'
3/10/2023NBA_9102_v109_revO_09Jul2020EnglishMinION
February 2023 - Updated product phase to 'Legacy'
August 10th, 2022NBA_9102_v109_revN_09Jul2020EnglishGridion Download 1st August 2022 - Removed references to FFPE in the End-prep step. - Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
August 10th, 2022NBA_9102_v109_revN_09Jul2020EnglishMinion Download 1st August 2022 - Removed references to FFPE in the End-prep step. - Updated the steps for preparing third party reagents in 'Native barcode ligation' and 'Adapter ligation and clean-up' steps.
July 14th, 2022NBA_9102_v109_revM_09Jul2020EnglishGridion Download 14th July 2022 - updated compabilities and removed references to discontinued flow cells
July 14th, 2022NBA_9102_v109_revM_09Jul2020EnglishMinion Download 14th July 2022 - updated compabilities and removed references to discontinued flow cells
July 4th, 2022NBA_9102_v109_revL_09Jul2020EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
July 4th, 2022NBA_9102_v109_revL_09Jul2020EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 14th, 2022NBA_9102_v109_revK_09Jul2020EnglishGridion Download 14th April 2022 Protocol name change
April 14th, 2022NBA_9102_v109_revK_09Jul2020EnglishMinion Download 14th April 2022 Protocol name change
April 14th, 2022NBA_9102_v109_revK_09Jul2020EnglishAny Download 14th April 2022 Protocol name change
January 26th, 2022NBA_9102_v109_revJ_09Jul2020EnglishGridion Download 26th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
January 26th, 2022NBA_9102_v109_revJ_09Jul2020EnglishMinion Download 26th January 2022 - Updated mixing recommendations and adding tips regarding short term storage of the DNA library during the prep
October 15th, 2021NBA_9102_v109_revI_09Jul2020EnglishGridion Download October 2021 - Clarified R10.3 flow cell loading recommendations
October 15th, 2021NBA_9102_v109_revI_09Jul2020EnglishMinion Download October 2021 - Clarified R10.3 flow cell loading recommendations
September 14th, 2021NBA_9102_v109_revH_09Jul2020EnglishGridion Download 14th Sept 2021 - In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
September 14th, 2021NBA_9102_v109_revH_09Jul2020EnglishMinion Download 14th Sept 2021 - In "Overview of the protocol" and "Equipment and consumables", there is a note that the 96 barcode orientation on the plate differs depending on the kit batch number
September 13th, 2021NBA_9102_v109_revG_09Jul2020EnglishGridion Download 13th Sept 2021 - Added tables outlining kit contents to the Equipment and Consumables section.
September 13th, 2021NBA_9102_v109_revG_09Jul2020EnglishMinion Download 13th Sept 2021 - Added tables outlining kit contents to the Equipment and Consumables section.
April 21st, 2021NBA_9102_v109_revF_09Jul2020EnglishGridion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
April 21st, 2021NBA_9102_v109_revF_09Jul2020EnglishMinion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
January 22nd, 2021NBA_9102_v109_revE_09Jul2020EnglishGridion Download 11th Jan Updated mastermix volumes to volumes required per sample
January 22nd, 2021NBA_9102_v109_revE_09Jul2020EnglishMinion Download 11th Jan Updated mastermix volumes to volumes required per sample
January 4th, 2021NBA_9102_v109_revD_09Jul2020EnglishGridion Download 4th Jan Equipment list updated
January 4th, 2021NBA_9102_v109_revD_09Jul2020EnglishMinion Download 4th Jan Equipment list updated
December 8th, 2020NBA_9102_v109_revC_09Jul2020EnglishGridion Download 8th Dec Compatibilities updated to EXP-WSH004
December 8th, 2020NBA_9102_v109_revC_09Jul2020EnglishMinion Download 8th Dec Compatibilities updated to EXP-WSH004
November 9th, 2020NBA_9102_v109_revB_09Jul2020EnglishGridion Download 9th Nov Updated equipment and consumables
November 9th, 2020NBA_9102_v109_revB_09Jul2020EnglishMinion Download 9th Nov Updated equipment and consumables
August 19th, 2020NBA_9102_v109_revA_09Jul2020EnglishGridion Download 18th May Updated with corrections to RT step and Native Barcode Ligation
August 19th, 2020NBA_9102_v109_revA_09Jul2020EnglishMinion Download 18th May Updated with corrections to RT step and Native Barcode Ligation
August 19th, 2020NBA_9102_v109_revA_09Jul2020EnglishAny Download 18th May Updated with corrections to RT step and Native Barcode Ligation
July 7th, 2020NBD_9096_v109_revA_7JULY2020English- Download 18th May Updated with corrections to RT step and Native Barcode Ligation