Getting started guides
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A guide to biopharma solutions from Oxford Nanopore — from discovery to development
This guide introduces Oxford Nanopore sequencing solutions for biopharma that can be applied from biomarker discovery through to final product quality control (QC) testing.
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A guide to targeted sequencing with Oxford Nanopore
This guide provides an introduction to performing targeted nanopore sequencing.
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A guide to human genomics with Oxford Nanopore
This guide introduces how to sequence human genomes with Oxford Nanopore, from the construction of new, highly complete reference assemblies to the comprehensive identification of variants.
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A guide to investigating methylation in the human genome
This guide provides an introduction to the direct sequencing of DNA methylation in human genomes.
Workflow overviews
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Real-time enrichment of a comprehensive pharmacogenomic panel with Oxford Nanopore sequencing
This end-to-end workflow introduces how to enrich a comprehensive panel of PGx targets with real-time targeted Oxford Nanopore sequencing.
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Comprehensive characterisation of cancer predisposition genes using the Hereditary Cancer Panel
Discover the Hereditary Cancer Panel workflow and streamline your precision oncology research.
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Performing accurate species-level bacterial identification with nanopore sequencing
This end-to-end workflow provides a rapid solution for species-level bacterial identification.
Streamlined identification of bacteria, archaea, and fungi with Oxford Nanopore microbial amplicon barcoding
This end-to-end workflow introduces how to sequence the full length 16S gene and ITS region with Oxford Nanopore technology.
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Rapid viral sequencing for genomic surveillance of mpox using metagenomic or targeted approaches
This end-to-end workflow provides guidance for both metagenomic and targeted viral genome sequencing to deliver rapid and actionable results.
Most viewed
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Hereditary Cancer Panel: targeted sequencing via adaptive sampling
In this flyer, discover how to utilise the Oxford Nanopore Hereditary Cancer Panel to investigate 258 key genes associated with hereditary cancer risk.
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Comprehensive genomic and epigenomic profiling with the Oxford Nanopore Hereditary Cancer Panel
In this application note, we present the design and performance of the Oxford Nanopore Hereditary Cancer Panel targeting 258 key genes implicated in cancer predisposition.
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Go with the gold standard in methylation sequencing
In this flyer, find out how Oxford Nanopore provides gold-standard methylation sequencing and comprehensive variant calling in a single run — without chemical conversion.
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GridION Q: Oxford Nanopore sequencing designed for use in regulated and applied environments
Discover GridION Q, the sequencing solution designed for use in regulated and applied environments from Oxford Nanopore Technologies.
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Oxford Nanopore sequencing solutions for microbiology and infectious disease research
In this brochure, discover Oxford Nanopore sequencing techniques that can reveal multidimensional insights for your microbial research applications.
Featured resources
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Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
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Accelerating cancer research through comprehensive genomic analysis
Unlock previously hidden variation with nanopore sequencing and characterise cancer at unprecedented resolution.
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How do I perform real-time targeted Oxford Nanopore sequencing?
What if targeted sequencing took place during sequencing itself, without the need for special library prep, PCR, or probes? In this demo, discover Adaptive Sampling: a unique, real-time targeted enrichment/depletion method that achieves just that.
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Faster analysis of cancer-associated variants using a unique targeted nanopore sequencing approach
In this case study, discover the benefits of adaptive sampling and how it has been applied to cancer research.
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Which library prep workflow is right for my experiment?
Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow.
Protocols
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使用 SQK-RBK114(.24 或 .96)进行质粒 DNA 测序
本方案提供了最便捷高效的质粒 DNA 测序流程。
此实验指南:
- 支持多达96个不同样本的混合测序
- 文库制备用时约60分钟
- 产量高
- 包含片段化步骤
- 兼容 R10.4.1 测序芯片
仅供研究使用
Amplicon sequencing from DNA using SQK-RBK114 (.24 or .96)
This is the fastest and simplest protocol to sequence amplicon DNA.
For Research Use Only
Document version: RAA_9198_v114_revM_17Oct2025
Analysis workflows
wf-tb-amr
wf-tb-amr is a workflow for determining the antibiotic resistance of Mycobacterium tuberculosis targeted sequencing samples.
wf-mpx
This workflow provides a simple way to analyse mpox sequencing data; taking raw Oxford Nanopore Technologies reads and creating a draft consensus and assembly.
wf-flu
This analysis workflow can be used with Oxford Nanopore Technologies sequencing data from amplified segments of the Influenza Type A and Type B genomes, to determine the most likely strain of Influenza to which the sequenced sample belongs.
wf-cas9
The ONT Cas9 sequencing kit allows the enrichment of genomic regions of interest by amplifying target regions from adapters ligated to Cas9 cleavage sites. The purpose of this workflow is to assess the effectiveness of such Cas9 enrichment, but it can be applied to other enrichment approaches.
wf-bacterial-genomes
This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.
Latest research
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Nanopore sequencing combined with Adaptive Sampling and NanoExpansion enables accurate characterisation of repeat expansion disorders
Authors: Luca Morandi, Francesco Casadei, Sara De Fanti, Luigi Bonan, Francesco Ravaioli, Flavia Palombo, Alessia Fiorentino, Silvia de Pasqua, Veria Vacchiano, Patrizia Avoni, Giovanni Rizzo, Sabina Capellari, Edoardo Ruggeri, Vincenzo Donadio, Gastone Castellani, Maria Giulia Bacali
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Strengthening MRSA genomic surveillance in the Pacific
Discover how strengthening local sequencing and bioinformatics capacity can support sustainable pathogen surveillance and informed public health responses across the Pacific.
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Richer insights with adaptive sampling: targeted panels without probes for cancer predisposition, tumor profiling and beyond
Learn about Oxford Nanopore's off-the-shelf solutions for creating simple, rapid custom panels providing data-rich scalable insights into cancer biology.
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Variant phasing for antisense oligonucleotide design using adaptive sampling | LC 25
Joy Goffena is a Research Scientist in Dr Danny Miller’s lab at the University of Washington. She specializes in applying Oxford Nanopore Technologies sequencing to advance genetic research and clinical diagnostics. Her work focuses on identifying causal genetic variants...
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Long-read sequencing and adaptive sampling solve complex diagnostic conundrums | LC 25
Prof. Sebastian Lunke is the Head of Division of Genetics and Genomics at the Victorian Clinical Genetics Services. His team oversees the development and delivery of clinically accredited genetics and genomics laboratories in Australia, with a strong focus on translating...