Getting started guides
)
A guide to targeted sequencing with Oxford Nanopore
This guide provides an introduction to performing targeted nanopore sequencing.
Most viewed
)
PromethION 24: sequencing to the power of 24
Multiply your insights with PromethION 24, the production-scale nanopore sequencer that puts you in control of up to 24 high-output flow cells. Discover more.
Featured resources
)
Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
)
How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
)
Which library prep workflow is right for my experiment?
Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow. In this masterclass, discover: • The range of Oxford Nanopore library prep solutions av
)
How do I extract high-quality DNA and RNA for my Oxford Nanopore sequencing experiment?
Great sequencing results start with high-quality DNA and RNA extraction. In this masterclass, find out how to achieve just that — from choosing the right method for you to performing size selection, fragmentation, and sample handling. In this masterclass, discover: • How to optimise extraction for y
)
How do I get started with Oxford Nanopore sequencing?
Your Oxford Nanopore sequencing journey starts here. In this masterclass, find out how nanopore sequencing works, what to expect at each step of your workflow, and how to plan your first experiment. In this masterclass, discover: • How Oxford Nanopore sequencing technology works and its benefits for
Protocols
基因组DNA连接法测序 - 免扩增条形码测序试剂盒-24(SQK-NBD114.24)
本文档描述了基因组 DNA 的免扩增条形码建库流程。
实验指南:
- 使用免扩增条形码测序试剂盒-24 V14 (SQK-NBD114.24)
- 无需PCR扩增
- 支持多达24种条形码
- 可分析天然DNA
- 与R10.4.1 测序芯片兼容
仅供研究使用
基因组DNA连接法测序 - 免扩增条形码测序试剂盒-96(SQK-NBD114.96)
本文档描述了基因组 DNA 的免扩增条形码建库流程。
实验指南:
- 使用免扩增条形码测序试剂盒-96 V14 (SQK-NBD114.96)
- 无需PCR扩增
- 支持多达96种条形码
- 可分析天然DNA
- 与R10.4.1 测序芯片兼容
仅供研究使用
DNA连接法建库 V14(SQK-LSK114)
本实验指南:
- 使用基因组DNA或扩增子DNA为起始材料
- 建库用时约65分钟
- 用户可按需片段化DNA
- 无需借助PCR
- 与 R10.4.1 测序芯片兼容
仅供研究使用
Analysis workflows
wf-transcriptomes
Transcriptome analysis of cDNA and direct RNA sequencing data.
wf-pore-c
Workflow for analysing Pore-c data for chromatin conformation capture.
wf-cas9
The ONT Cas9 sequencing kit allows the enrichment of genomic regions of interest by amplifying target regions from adapters ligated to Cas9 cleavage sites. The purpose of this workflow is to assess the effectiveness of such Cas9 enrichment, but it can be applied to other enrichment approaches.
wf-alignment
This workflow provides an easy way to align Oxford Nanopore reads and gather mapping stats either locally for small amounts of data or at scale in a distributed environment such as a cluster or the cloud.
Latest research
)
DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage
Publication: DNA methylation reprogramming in marsupial embryos is restricted to the extraembryonic lineage
)
Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa
Publication: Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa
)
Swine influenza-modified pulmonary microbiota
Publication: Swine influenza-modified pulmonary microbiota
)
16S vs shotgun nanopore sequencing detected different taxa in the same freshwater samples
Problem: 16S and shotgun assembly and read-based approaches are widely used for taxonomic profiling. The resulted taxonomic assignments are compared across studies. We showed that the estimated taxa are biased by the choice of shotgun vs amplicon method. Methods: Target database: GTDB Re
)
A high-quality Oxford Nanopore assembly of the hourglass dolphin (Lagenorhynchus cruciger) genome
Publication: A high-quality Oxford Nanopore assembly of the hourglass dolphin (Lagenorhynchus cruciger) genome