Fusion transcripts
Getting started guides
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A guide to transcriptomics with Oxford Nanopore
This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.
Workflow overviews
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Detecting isoforms and RNA modifications with PCR-free, direct RNA nanopore sequencing
This end-to-end workflow provides a simple method to characterise RNA modifications from a human blood research sample using direct RNA sequencing.
Most viewed
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Long cDNA sequencing reads enable transcriptome analysis at isoform resolution
In this application note, we evaluated the updated Oxford Nanopore cDNA-PCR sequencing protocol updated protocol and compared its performance with that of the previous version of the protocol, Illumina short-read RNA-seq, and Pacific Biosciences Kinnex.
Featured resources
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Unlocking RNA biology with full-length reads
Discover how nanopore sequencing uniquely captures full-length transcripts for isoform-level transcriptome characterisation.
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Accelerating cancer research through comprehensive genomic analysis
Unlock previously hidden variation with nanopore sequencing and characterise cancer at unprecedented resolution.
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How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
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How do I load a PromethION Flow Cell?
Preparing and loading a PromethION Flow Cell is straightforward. In this demo, we walk you through the process step by step. In this masterclass, discover: • How real-time Oxford Nanopore sequencing produces data • How to use MinKNOW to monitor your sequencing run, basecall your data, and perform re
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How do I load a MinION Flow Cell?
Preparing and loading a MinION Flow Cell is straightforward. In this demo, we walk you through the process step by step.
Protocols
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直接RNA测序试剂盒（SQK-RNA004）
本实验指南：
- 用于为天然RNA测序
- 可以总RNA或富集样本（如带polyA尾的RNA或去除rRNA的RNA）为起始材料
- 无需片段化
- 建库需约140分钟
- 仅与RNA测序芯片兼容
仅供研究使用
Analysis workflows
Latest research
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Long-read single-cell genome, transcriptome and open chromatin profiling links genotype to phenotypes
Publication: Long-read single-cell genome, transcriptome and open chromatin profiling links genotype to phenotypes
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Rapid epigenomic classification of acute leukaemia
Publication: Rapid epigenomic classification of acute leukaemia
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A founder BRCA1 exonic duplication involving breakpoint in T2T reference genome-specific region results in constitutional fusion transcript
Publication: A founder BRCA1 exonic duplication involving breakpoint in T2T reference genome-specific region results in constitutional fusion transcript
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Advancing long-read nanopore genome assembly and accurate variant calling for rare disease detection
Publication: Advancing long-read nanopore genome assembly and accurate variant calling for rare disease detection
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An atlas of expressed transcripts in the prenatal and postnatal human cortex
Publication: an atlas of expressed transcripts in the prenatal and postnatal human cortex
