Splice variation
Getting started guides
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A guide to transcriptomics with Oxford Nanopore
This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.
Workflow overviews
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Detecting isoforms and RNA modifications with PCR-free, direct RNA nanopore sequencing
This end-to-end workflow provides a simple method to characterise RNA modifications from a human blood research sample using direct RNA sequencing.
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Obtaining full-length isoforms from single cells with Oxford Nanopore sequencing
This end-to-end workflow provides a simple solution for single-cell transcriptome analysis from 10x Genomics cDNA with library preparation in approximately three hours.
Featured resources
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Powered by long nanopore reads, liver transcriptome analysis reveals new clues about cancer
Just as short-read RNA-seq technology revealed gene expression data that was not possible with previous methods such as microarrays, long nanopore reads are now unlocking deeper insights into transcriptomics that were previously out of reach. The assembly process required to stitch short reads ba
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Nanopore sequencing improves the characterisation of mutations driving blood cancer
With recent advances in drug development, especially in the immunotherapy field, it has never been more important to fully elucidate the biological hallmarks and mechanisms of cancer. Scientists are deploying many different tools to answer this need. In two papers, research teams described new techn
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Mapping RNA modifications in the human brain with full-length transcript sequencing
A single gene can produce multiple RNA isoforms through complex gene regulation mechanisms, such as alternative splicing and post-transcriptional modifications. This creates a large transcriptional diversity by generating multiple isoforms, as exhibited in the human brain, facilitating the regulatio
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Update from Oxford Nanopore Technologies
Clive Brown is CTO of Oxford Nanopore, where he leads the innovation of breakthrough technologies and product development, that are designed to enable the analysis of anything, by anyone, anywhere.
Clive has held a variety of roles in Genomics and Genetic R&D, he also played a key role...
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London Calling 2023: Direct RNA sequencing update (SQK-RNA004): initial impressions of RNA004 and its application for functional profiling of cancer-associated long non-coding RNAs
Oxford Nanopore Technologies’ direct RNA sequencing chemistry is unique as it determines the sequence of long-read RNA transcripts, capturing full-length isoforms, UTRs, and poly(A) tail lengths, without the requirement of first converting to DNA, nor PCR amplification. Here, we present our new, imp
Protocols
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直接RNA测序试剂盒（SQK-RNA004）
本实验指南：
- 用于为天然RNA测序
- 可以总RNA或富集样本（如带polyA尾的RNA或去除rRNA的RNA）为起始材料
- 无需片段化
- 建库需约140分钟
- 仅与RNA测序芯片兼容
仅供研究使用
Analysis workflows
wf-aav-qc
This workflow takes reads sequenced from adeno-associated virus (rAAV) vector preps and does some basic quality control checks.
Latest research
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Exon skipping as a potential diagnostic biomarker in colorectal cancer: an integrated epigenomic-transcriptomic analysis
Publication: Exon skipping as a potential diagnostic biomarker in colorectal cancer: an integrated epigenomic-transcriptomic analysis
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Long-read spatial transcriptomics of patient-derived clear cell renal cell carcinoma organoids identifies heterogeneity and transcriptional remodelling following NUC-7738 treatment
Publication: Long-read spatial transcriptomics of patient-derived clear cell renal cell carcinoma organoids identifies heterogeneity and transcriptional remodelling following NUC-7738 treatment
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Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
Publication: Transcriptional readthrough precedes alternative splicing programs triggered in CML cells by imatinib
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Deep intronic SVA_E insertion identified as the most common pathogenic variant associated with Canavan disease: a diagnostic blind spot
Publication: Deep intronic SVA_E insertion identified as the most common pathogenic variant associated with Canavan disease: a diagnostic blind spot
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A founder BRCA1 exonic duplication involving breakpoint in T2T reference genome-specific region results in constitutional fusion transcript
Publication: A founder BRCA1 exonic duplication involving breakpoint in T2T reference genome-specific region results in constitutional fusion transcript
