Gene expression
Getting started guides
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A guide to transcriptomics with Oxford Nanopore
This guide introduces cDNA and direct RNA Oxford Nanopore sequencing, for ultra-rich transcriptomic data without compromise.
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A guide to single-cell transcriptomics with Oxford Nanopore
This guide introduces the single-cell RNA nanopore sequencing workflow, for ultra-rich data without compromise.
Workflow overviews
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Detecting isoforms and RNA modifications with PCR-free, direct RNA nanopore sequencing
This end-to-end workflow provides a simple method to characterise RNA modifications from a human blood research sample using direct RNA sequencing.
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Sequencing full-length transcripts for isoform-level expression analysis
This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis.
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Obtaining full-length isoforms from single cells with Oxford Nanopore sequencing
This end-to-end workflow provides a simple solution for single-cell transcriptome analysis from 10x Genomics cDNA with library preparation in approximately three hours.
Most viewed
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cDNA-PCR Sequencing Kits
In this flyer, discover how you can sequence full-length transcripts and annotate the transcriptome with confidence.
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Long cDNA sequencing reads enable transcriptome analysis at isoform resolution
In this application note, we evaluated the updated Oxford Nanopore cDNA-PCR sequencing protocol updated protocol and compared its performance with that of the previous version of the protocol, Illumina short-read RNA-seq, and Pacific Biosciences Kinnex.
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EPI2ME: data analysis for all levels of expertise
Discover EPI2ME and how it provides data analysis for all levels of expertise
Featured resources
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Leveraging base modification information with Modkit | LC26
Nanopore sequencing reads from native DNA and RNA are inherently multiomic. However, using this data with confidence can be daunting. I will show how improvements in machine learning models have made it easier than ever to acquire this data. Then we will touch on best practices for a variety of exam
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Epitranscriptomic regulation of the developing human pancreas | LC26
RNA modifications are crucial regulators of gene expression in human development. They have an established role in regulating pancreatic β-cell development and function, with dysregulation resulting in diabetes. Numerous limitations have restricted the study of these modifications directly in pancre
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The long and short of it
Kicking off the second plenary of NCM 2019 was Sissel Juul, Director of Genomic Application at Oxford Nanopore. Sissel began by introducing the applications team: in Oxford, United Kingdom, the group focus on sample technology, whilst the Genomic Applications team is split across Oxford, New York an
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Update from Oxford Nanopore Technologies
Clive Brown is CTO of Oxford Nanopore, where he leads the innovation of breakthrough technologies and product development, that are designed to enable the analysis of anything, by anyone, anywhere.
Clive has held a variety of roles in Genomics and Genetic R&D, he also played a key role...
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Expanding reference genomes with T2T assembly to reflect global diversity
Discover how researchers are utilising Oxford Nanopore data to generate telomere-to-telomere (T2T) genomes, addressing the critical gaps in reference genomes.
Protocols
cDNA-PCR Sequencing V14 (SQK-PCS114)
cDNA-PCR Sequencing V14 (SQK-PCS114)
cDNA-PCR 测序 V14 - 条形码文库构建（SQK-PCB114.24）
本文档介绍了一种最便捷高效的全长 cDNA 测序流程。
该流程具有以下特点：
- 实现最高测序产量
- 产量优于传统的 cDNA 合成方法
- 可检测剪接变异及融合转录本
- 支持多达24个不同样本的混合测序
- 仅兼容 R10.4.1 测序芯片 仅供研究使用
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直接RNA测序试剂盒（SQK-RNA004）
本实验指南：
- 用于为天然RNA测序
- 可以总RNA或富集样本（如带polyA尾的RNA或去除rRNA的RNA）为起始材料
- 无需片段化
- 建库需约140分钟
- 仅与RNA测序芯片兼容
仅供研究使用
DNA连接法建库 V14（SQK-LSK114）
本实验指南：
- 使用基因组DNA或扩增子DNA为起始材料
- 建库用时约65分钟
- 用户可按需片段化DNA
- 无需借助PCR
- 与 R10.4.1 测序芯片兼容
仅供研究使用
Latest research
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A complete diploid human genome benchmark for personalised genomics
Publication: A complete diploid human genome benchmark for personalised genomics
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A single amino acid variant in the variable region I of AAV capsid confers liver de-targeting
Publication: A single amino acid variant in the variable region I of AAV capsid confers liver de-targeting
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A spatial long-read approach at near-single-cell resolution reveals developmental regulation of splicing and polyadenylation sites in distinct cortical layers and cell types
Publication: A spatial long-read approach at near-single-cell resolution reveals developmental regulation of splicing and polyadenylation sites in distinct cortical layers and cell types
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Advancing long-read nanopore genome assembly and accurate variant calling for rare disease detection
Publication: Advancing long-read nanopore genome assembly and accurate variant calling for rare disease detection
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