Splice variation
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Capture full-length transcript isoforms in single reads
The identification of differentially spliced RNA isoforms, and their functional effects, is of high importance in the study of both healthy variation and disease, with aberrant splicing implicated in diseases including cancer and neurological disorders.
Legacy short-read RNA-Seq methods typically cannot span full-length transcript isoforms, requiring them to be computationally reassembled; this can lead to incorrect reconstruction. With Oxford Nanopore reads of unrestricted length, isoforms can be sequenced end to end in single reads, enabling their unambiguous characterisation — and simultaneous quantification, in a single dataset.
Featured
The value of full-length transcripts without bias
This white paper introduces the facility of RNA and cDNA nanopore sequencing to deliver full-length transcript information, isoform characterisation and quantification, and RNA virus identification.
Direct RNA sequencing workflow
In this workflow, discover how to characterise full-length splice isoforms and RNA modifications with direct RNA nanopore sequencing.
Recommended device for splice variant detection
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PromethION 24
Providing capacity to sequence on up to 24 independently addressable PromethION Flow Cells and powerful onboard compute, the PromethION 24 delivers high-coverage nanopore sequencing of whole transcriptomes for RNA isoform identification and quantification.
