Splice variation
The identification of differentially spliced isoforms, and their functional effects, is of high importance in the study of both healthy variation and disease, with aberrant splicing implicated in diseases including cancer and neurological disorders. However, traditional short-read RNA-Seq methods typically cannot span full-length isoforms, requiring them to be computationally reassembled; this can lead to incorrect reconstruction. With long nanopore reads, isoforms can be sequenced end-to-end in single reads, enabling their unambiguous characterisation — and simultaneous quantification, in a single dataset.
Accurately resolve and quantify full-length splice variants with long sequencing reads
Identify epigenetic modifications alongside nucleotide sequence through direct RNA sequencing
Scale to your output requirements with a range of sequencing platforms
What is alternative splicing?
Gene splicing is an important mechanism that regulates isoform expression in a cell-specific or timing-specific (e.g. during development) manner. Different categories of alternative splicing have been described, including exon skipping, the most frequently occurring type, and intron retention (Figure 1). It is estimated that 95% of multiexon genes are alternatively spliced in humans, with an average of three transcripts produced per geneMathur, M. et al. Programmable mutually exclusive alternative splicing for generating RNA and protein diversity Nat. Commun. 10, 2673 (2019).
Alternative splicing can have dramatic effects on the proteins produced. For example, protein-protein interactions may be altered, enzyme function inhibited, or location of expression may be changed (e.g. a cell-surface protein may instead be secreted). Such splice variation may significantly impact disease riskScotti, M. and Swanson, M. RNA mis-splicing in disease. Nat. Rev. Genet.17, 19–32 (2016), disease progression, and even drug responsesGregory, A. et al. TNF receptor 1 genetic risk mirrors outcome of anti-TNF therapy in multiple sclerosis. Nature 488, 508–511 (2012).
RNA splicing was first discovered by analysis of adenovirus RNA arrangement in the late 1970sBerget, S.M., Moore. C., and Sharp, P.A. Spliced segments at the 5' terminus of adenovirus 2 late mRNA. Proc. Natl. Acad. Sci. USA 74,8: 3171-5 (1977), Chow, L.T. et al. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA. Cell. 12(1):1-8 (1977). In contrast to the larger genomes of humans, plants, and animals, splicing enables efficient use of the small genomes of viruses. In adenoviruses, differential splicing controls gene expression, and therefore protein production, across the different stages of infection.
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Figure 1. Six alternative splicing categories. Exons are represented by a coloured box and introns by a black horizontal line. For each category, the different splicing reactions are symbolised by a red line. For the alternative 5′ splice site (ss) or 3′ ss, the use of the upstream 5′ ss or the downstream 3′ ss generates a shorter upstream or downstream exon, respectively.
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Figure 2. Alternative splicing can produce numerous isoforms per gene. A Drosophila melanogaster transcriptome dataset was created, and isoforms were reconstructed. Compared to the reference isoform set from Ensembl, high exon and transcript-level precision can be seen.
Accurately characterise splice variation with long nanopore sequencing reads
Comprehensive analysis of splice variation is limited with short-read sequencing; although exon junctions can be observed, resolution of entire isoforms is extremely challenging. With nanopore sequencing, read length is equal to fragment length, meaning entire transcripts can be sequenced in single reads. Full-length transcripts >20 kb in length have been sequenced in single reads. This greatly simplifies the identification and quantification of entire isoforms (Figure 2).
In addition to analysis of cDNA molecules, nanopore technology uniquely enables direct sequencing of native RNA molecules. This allows base modification information to be obtained alongside nucleotide sequence data; no additional sample preparation or sequencing runs are needed to acquire epigenetic data. Investigating the association of methylation with splice variation is therefore greatly simplified with Oxford Nanopore sequencing technology. As an example, splicing regulation by N6-methyladenosine (m6A) modification was recently described in adenovirus Price, A. M.et al. Direct RNA sequencing reveals m6A modifications on adenovirus RNA are necessary for efficient splicing. bioRxiv 865485 (2019). With nanopore direct RNA sequencing, m6A modifications could be identified at the nucleotide level, and as the overlapping splice units could be mapped precisely with long reads, methylation could also be accurately resolved at the transcript level.
Trancriptome variation in human tissues
The advent of long-read sequencing technologies offers the opportunity to study the role of genetic variation in transcript structure
Glinos et al. Nature 608: 353–359 (2022)
Discover how Glinos et al. are using long nanopore reads to investigate how splicing variants could affect transcript structure and disease risk.
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Targeted sequencing of full-length transcripts reveals isoform diversity across human neurodevelopment
At NCM 2022, Rosemary Bamford (University of Exeter, UK) reported on her work characterising alternative splicing in the cerebral cortex of mice and humans. Using targeted nanopore transcript sequencing, her team were able to identify differentially expressed genes and alternative splicing in the brain, and obtain an ultra-deep view of transcripts during neurodevelopment.
Understanding the mechanisms of RNA processing using direct RNA nanopore sequencing
Newly synthesised messenger RNAs (mRNAs) undergo several processing steps prior to their export to the cytoplasm. At NCM 2022, Karine Choquet (Harvard Medical School, USA) presented her work exploring the landscape of full-length mRNA isoforms across different subcellular compartments, using direct RNA nanopore sequencing of poly(A)-selected RNA from whole-cell, chromatin, cytoplasm, and polysome fractions in human cells. In her talk, she described the first transcriptome-wide characterisation of splicing and polyadenylation across long mRNA isoforms in distinct subcellular compartments.
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How do I perform alternative splicing analysis using nanopore sequencing?
Oxford Nanopore provides three RNA sequencing kits that can be used for gene expression and downstream splice variation analysis, all of which deliver full-length transcripts. The choice of kit depends on your specific study requirements, including sample amounts, requirement for sample multiplexing, base modification detection, and desired number of reads.
The range of nanopore sequencing devices enables you to scale according to your throughput and output requirements, from the portable Flongle and MinION devices, which are well suited to targeted splice variation analysis, to the modular GridION, high-output PromethION 2 devices, and ultra-high-throughput, high-output PromethION P24 and PromethION 48 devices, ideal for transcriptome-wide investigations.
Oxford Nanopore provides a wide range of intuitive EPI2ME workflows to support nanopore data analysis for all levels of expertise.
Splice variation analysis in the human transcriptome
Library preparation with the cDNA-PCR Sequencing Kit, followed by sequencing on a PromethION device, is ideal for transcriptome-wide analysis of splice variation. Multiplexing of up to 24 samples can be achieved using the cDNA-PCR Barcoding Kit.
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Interested in RNA modifications?
Check out the Direct RNA Sequencing Kit to identify RNA modifications alongside nucleotide sequence — in a single assay.
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