Gene expression
Full-length transcripts in single reads
Analysis of gene expression is important in many applications, from clinical research to developmental biology. However, the use of legacy short-read technologies can cause multi-mapping when aligning data and limit quantification accuracy; this can be further limited by PCR bias.
In contrast, Oxford Nanopore reads of unrestricted length allow transcripts to be sequenced end to end, enabling accurate quantification and complete characterisation of isoforms in a single dataset. Furthermore, direct RNA sequencing enables the simultaneous detection of epigenetic modifications and eliminates PCR bias.
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Resolve gene expression at isoform resolution with long cDNA reads
In this application note, we evaluated the latest Oxford Nanopore cDNA-PCR sequencing protocol, demonstrating distinct advantages over the previous protocol, Illumina RNA-seq, and PacBio Kinnex.
The value of full-length transcripts without bias
This white paper focuses on the facility of nanopore RNA and cDNA sequencing to tackle challenges in the areas of full-length transcript identification, isoform characterisation and quantification, and viral detection.
High-definition transcriptomics
Recommended device for gene expression studies
PromethION 2 Integrated and 2 Solo
Gain flexible, high-output nanopore sequencing for every lab with the PromethION 2 devices. Generate high depth of coverage of whole transcriptomes on up to two individually addressable PromethION Flow Cells, enabling high-resolution gene expression analysis.
The PromethION 2 Integrated is a self-contained, benchtop device with powerful onboard compute, while the compact PromethION 2 Solo utilises resource within a GridION or other existing compute.
