Getting started guides
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A guide to microbial sequencing with Oxford Nanopore
A guide to get started with sequencing microbial samples with Oxford Nanopore.
Most viewed
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GridION: one sequencer for many
Made with multiple projects and teams in mind, GridION gives you the freedom to start, stop, and scale your sequencing experiments without impacting others. Meanwhile, onboard compute keeps your real-time data optimised. Discover more.
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MinION: palm sized, world ready
Discover how the portable yet powerful MinION Mk1D sequencing device can take your insights further afield. Start generating rapid, real-time results without compromise.
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Oxford Nanopore sequencing solutions for microbiology and infectious disease research
Discover the Oxford Nanopore sequencing techniques that can achieve comprehensive microbial genome characterisation.
Workflow overviews
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Nanopore-only microbial isolate sequencing solution (NO-MISS): flexible, rapid bacterial and fungal isolate whole-genome sequencing
This end-to-end workflow provides a rapid solution for whole-genome Oxford Nanopore sequencing of bacterial and fungal isolates.
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Performing accurate species-level bacterial identification with nanopore sequencing
This end-to-end workflow provides a rapid solution for species-level bacterial identification.
Featured resources
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Genomics for a changing planet: sequencing the living world
Discover how a global community of researchers are harnessing Oxford Nanopore sequencing to study the far-reaching impacts of climate change, revealing deep insights across environmental research, agriculture, and pathogen surveillance.
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Addressing the challenges of metagenomics with Oxford Nanopore sequencing
Explore how reads with unrestricted length are revealing unprecedented insight into microbial communities.
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From full-length 16S to high-resolution metagenomics: how do I choose the right microbial community workflow for my experiment?
In this masterclass, find the right microbial community sequencing workflow to meet your experimental goals. We’ll cover microbial identification from full-length 16S/ITS sequencing through to comprehensive metagenomic assembly. In this masterclass, discover: • How Oxford Nanopore sequencing deliver
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How do I analyse my Oxford Nanopore sequencing data without specialist training?
In this beginner-friendly masterclass, discover how to analyse your Oxford Nanopore sequencing data. Find out how basecalling works, the common file formats you’ll encounter, and how to use MinKNOW to set up and monitor your sequencing run. Then, discover how to analyse your data with EPI2ME — no pr
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Which library prep workflow is right for my experiment?
Whether you’re sequencing DNA, RNA, or cDNA, a single sample or many in multiplex, there’s an Oxford Nanopore library prep kit for you. In this masterclass, discover how to choose the right one for your workflow. In this masterclass, discover: • The range of Oxford Nanopore library prep solutions av
Protocols
Rapid sequencing DNA - PCR Barcoding Kit 24 V14 (SQK-RPB114.24)
This protocol:
Uses genomic DNA
Has a low input requirement
Has a method involving tagmentation, barcoding and PCR amplification
Allows multiplexing of 1–24 samples
Is compatible with R10.4.1 flow cells
For Research Use Only
快速DNA测序V14 - 条形码(SQK-RBK114.24 或 SQK-RBK114.96)
本实验指南:
- 使用基因组DNA
- 支持为1-96个样本混样测序
- 建库用时仅需约60分钟
- 产出量高
- 包含片段化步骤
- 与R10.4.1 测序芯片兼容
仅供研究使用
DNA连接法建库 V14(SQK-LSK114)
本实验指南:
- 使用基因组DNA或扩增子DNA为起始材料
- 建库用时约65分钟
- 用户可按需片段化DNA
- 无需借助PCR
- 与 R10.4.1 测序芯片兼容
仅供研究使用
基因组DNA连接法测序 - 免扩增条形码测序试剂盒-24(SQK-NBD114.24)
本文档描述了基因组 DNA 的免扩增条形码建库流程。
实验指南:
- 使用免扩增条形码测序试剂盒-24 V14 (SQK-NBD114.24)
- 无需PCR扩增
- 支持多达24种条形码
- 可分析天然DNA
- 与R10.4.1 测序芯片兼容
仅供研究使用
基因组DNA连接法测序 - 免扩增条形码测序试剂盒-96(SQK-NBD114.96)
本文档描述了基因组 DNA 的免扩增条形码建库流程。
实验指南:
- 使用免扩增条形码测序试剂盒-96 V14 (SQK-NBD114.96)
- 无需PCR扩增
- 支持多达96种条形码
- 可分析天然DNA
- 与R10.4.1 测序芯片兼容
仅供研究使用
Analysis workflows
wf-metagenomics
This workflow can be used for the taxonomic classification of metagenomic sequencing data.
wf-bacterial-genomes
This workflow is primarily used to assemble genomes from bacterial reads and provide information on features of interest within those assemblies through annotations.
wf-amplicon
This Nextflow workflow provides a simple way to analyse Oxford Nanopore reads generated from haploid amplicons.
wf-alignment
This workflow provides an easy way to align Oxford Nanopore reads and gather mapping stats either locally for small amounts of data or at scale in a distributed environment such as a cluster or the cloud.
wf-16s
This workflow can be used for taxonomic classification of single reads from amplicon-targeted sequencing.
Latest research
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Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa
Publication: Decoding the Peruvian Amazon with in situ DNA barcoding of vertebrate and plant taxa
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Barcode 100K specimens: in a single nanopore run
Publication: Barcode 100K specimens: in a single nanopore run
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Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
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Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
Publications: Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires
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