Target enrichment nanopore sequencing with adaptive sampling can determine the structure of the small supernumerary marker chromosomes
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Structural analysis of small supernumerary marker chromosomes (sSMCs) has revealed many sSMCs carrying complex chromosome structures with multiple fragments. However, structural analysis of sSMCs by short-read sequencing is inefficient because most sSMCs are mosaic and consist of a small region of the involved chromosome. In this study, we applied adaptive sampling using long-nanopore sequencing reads to efficiently enrich the target region and determine the structure of sSMCs.