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RNA sequencing white paper: The value of full-length transcripts without bias

This white paper outlines how nanopore sequencing technology uniquely delivers high yields of long, full-length RNA, supporting quantification and complete transcriptome characterisation at the isoform level. Nanopore sequencing allows the generation of novel insights into transcript isoform expression and usage across different experimental conditions and cells, including single-cell analysis.

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RNA sequencing (referred to as RNA-Seq with traditional sequencing technologies) has led to unprecedented advances in all fields of biology and medicine. It has been an invaluable tool for the study of human genetics and the pathology associated with disease. Transcript isoform expression and usage, for example, is a prominent source of variation between healthy and diseased tissues in a number of medical conditions, including cancer. RNA sequencing is also instrumental in identifying fusion transcripts present in a growing number of disorders.

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