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Solutions for library preparation

Oxford Nanopore provides a comprehensive range of DNA and RNA library preparation kits, offering streamlined access to the benefits of short and long-fragments, real-time nanopore sequencing.

  • Short and long fragments, full-length reads and read length control
  • Rapid, streamlined protocols
  • Direct, amplification-free approaches — reducing hands-on time and preserving base modifications
  • Low input options
  • Sample multiplexing for cost-efficient results
  • Manual and automated for all sample throughput

Whole genome DNA sequencing kits

A wide range of library preparation kits are available to suit all whole genome sequencing requirements. Amplification-free kits allow direct, long-read sequencing of native DNA, eliminating the potential for PCR bias and allowing the detection of base modifications alongside nucleotide sequence. Amplification-based kits are also available, enabling whole genome sequencing from low input amounts or poor quality DNA (e.g. FFPE).

  Amplification-free, native DNA sequencing and retained base modification Amplification-based for low DNA amounts or quality
  Ligation Sequencing Kit Rapid Sequencing Kit Ultra-Long DNA Sequencing Kit Rapid PCR Barcoding Kit
Preparation time 60 min 10 min 60 min + ON elution 15 min + PCR
Input requirement 1000 ng of gDNA or 100-200 fmol of amplicons or cDNA 100-400 ng HMW gDNA
  • Tissue culture cells: 6 million
  • Blood: 1-2 ml
  • Gram-positive and -negative bacteria: ~2 ml of OD1 culture
1-5 ng gDNA
Fragmentation Optional Transposase based Transposase based Transposase based
Read length Equal to fragment length Random distribution, dependent on input fragment length 50-100+ kb N50 (R9.4.1 flow cells) ~2-5 kb
Typical throughput 3 2 2 2
Multiplexing options Yes Yes In development This kit offers barcoding for up to twelve samples
Overview Optimised for throughput; retained base modifications; control over read length; compatible with whole genome amplification Simple and rapid; retained base modifications; cold chain-free (Field kit) Optimised for production of ultra-long reads (N50 ≥50 kb); retained base modifications Simple and rapid
View all DNA sequencing kits

Targeted DNA sequencing kits

Long-read nanopore sequencing expands the application of targeted sequencing to include cost-effective, assembly-free analysis of repetitive regions, structural variants, and variant phasing across entire genes or large genomic regions of interest. Through analysis of the entire 16S rRNA gene, the long sequencing reads provided by the 16S Barcoding Kit also provide more accurate taxonomic classification of bacteria and archaea.

  Ligation Sequencing Kit Cas9 Sequencing Kit 16S Barcoding Kit
Approach Amplicon Sequence capture CRISPR/Cas9 Amplicon
Preparation time 60 min >24 hours 110 min 10 min + PCR
Input requirement 100-200 fmol amplicon 3.5 µg HMW gDNA 1-10 µg HMW gDNA (5 µg recommended) <10 ng gDNA
Fragmentation N/A Optional Cas9-dependent cleavage N/A
Read length Equal to fragment length Equal to fragment length post-PCR Equal to fragment length Full-length 16S gene (~1.5kb)
Typical throughput
Multiplexing options Yes No No Yes
Overview Simple, high-throughput workflow for existing amplicons Compatible with sequence capture approaches Streamlined workflow; preservation of base modifications Precise, real-time identification of bacteria and archaea

View all DNA sequencing kits

RNA sequencing kits

Confidently characterise and quantify full-length RNA transcripts, splice variants, and fusions using long-read nanopore sequencing. Accurately analyse differential gene expression and transcript usage. Sequence native RNA directly, without amplification or reverse transcription, and identify base modifications.

  Direct RNA Sequencing Kit cDNA-PCR Sequencing Kit
Preparation time 105 min 165 min
Input requirement 500 ng total RNA or 50ng poly-A+ RNA 4 ng poly(A)+ RNA or 200 ng total RNA
Reverse transcription required Optional Yes
PCR required No Yes
Read length Equal to RNA length Enriched for full-length cDNA
Typical throughput
Multiplexing options In development Yes
Overview Sequencing RNA molecules directly; identify base modifications and poly-A tail length Optimised for throughput
View all RNA sequencing kits