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DNA and RNA sequencing kits

Oxford Nanopore provides a comprehensive range of DNA and RNA library preparation kits, offering streamlined access to the benefits of long-read, real-time nanopore sequencing.

  • Long, full-length reads and read length control
  • Direct, amplification-free approaches — reducing hands-on time and preserving base modifications
  • Rapid, streamlined protocols
  • Cold chain-free Field Sequencing Kit
  • Low input options
  • Sample multiplexing for cost-efficient results

Whole genome DNA sequencing kits

A wide range of library preparation kits are available to suit all whole genome sequencing requirements. Amplification-free kits allow direct, long-read sequencing of native DNA, eliminating the potential for PCR bias and allowing the detection of base modifications alongside nucleotide sequence. Amplification-based kits are also available, enabling whole genome sequencing from low input amounts or poor quality DNA (e.g. FFPE).

  Amplification-free, native DNA sequencing and retained base modification Amplification-based for low DNA amounts or quality
  Ligation Sequencing Kit Rapid / Field Sequencing Kit PCR Sequencing Kit Rapid PCR Sequencing Kit
Preparation time 60 min 10 min 60 min + PCR 15 min + PCR
Input requirement 1,000 ng HMW gDNA 400 ng HMW gDNA 100 ng 10 ng
Fragmentation Optional Transposase based N/A Transposase based
Read length Equal to fragment length Random distribution, dependent on input fragment length Equal to fragment length post-PCR ~2-5 kb
Typical throughput
Multiplexing options Yes Yes Yes This kit offers barcoding for up to twelve samples
Overview Optimised for throughput; retained base modifications; control over read length; compatible with whole genome amplification Simple and rapid; retained base modifications; cold chain-free (Field kit) Control over read length Simple and rapid
View all DNA sequencing kits

Targeted DNA sequencing kits

Long-read nanopore sequencing expands the application of targeted sequencing to include cost-effective, assembly-free analysis of repetitive regions, structural variants, and variant phasing across entire genes or large genomic regions of interest. Through analysis of the entire 16S rRNA gene, the long sequencing reads provided by the 16S Barcoding Kit also provide more accurate taxonomic classification of bacteria and archaea.

  Ligation Sequencing Kit 16S Barcoding Kit
Approach Amplicon Sequence capture CRISPR/Cas9 Amplicon
Preparation time 60 min >24 hours 100 min 10 min + PCR
Input requirement 100-200 fmol amplicon 3,500 ng HMW gDNA 5,000 ng HMW gDNA <10 ng gDNA
Fragmentation N/A Optional Cas9 dependent cleavage N/A
Read length Equal to fragment length Equal to fragment length post-PCR Dependent on probe design Full-length 16S gene (~1.5kb)
Typical throughput
Multiplexing options Yes No No Yes
Overview Simple, high-throughput workflow for existing amplicons Compatible with sequence capture approaches Streamlined workflow; preservation of base modifications Precise, real-time identification of bacteria and archaea

View all DNA sequencing kits

RNA sequencing kits

Confidently characterise and quantify full-length RNA transcripts, splice variants, and fusions using long-read nanopore sequencing. Accurately analyse differential gene expression and transcript usage. Sequence native RNA directly, without amplification or reverse transcription, and identify base modifications.

  Direct RNA Sequencing Kit cDNA-PCR Sequencing Kit Direct cDNA Sequencing Kit
Preparation time 105 min 165 min 275 min
Input requirement 500 ng RNA (poly-A+) 1 ng RNA (poly-A+) 100 ng RNA (poly-A+)
Reverse transcription required Optional Yes Yes
PCR required No Yes No
Read length Equal to RNA length Enriched for full-length cDNA Enriched for full-length cDNA
Typical throughput
Multiplexing options In development Yes Yes
Overview Sequencing RNA molecules directly; identify base modifications and poly-A tail length Optimised for throughput No PCR bias
View all RNA sequencing kits

Sample multiplexing Run multiple DNA or RNA samples on a single flow cell — maximising flow cell usage and reducing cost per sample. Buy specific barcoding kits or enhance your existing native or amplification-based nanopore sequencing kit with an expansion pack. View all barcoding kits and expansion packs

Automate your sample prep VolTRAX is a portable, USB-powered device designed to automate sample and library prep for nanopore analyses. Discover VolTRAX
Protocol builder Optimise your complete nanopore sequencing workflow – from extraction to analysis. Build your protocol
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